| Literature DB >> 10649624 |
S Wang1, X Liu, K Fisher, J G Smith, F Chen, T W Tobery, J B Ulmer, R K Evans, M J Caulfield.
Abstract
DNA vaccines induce protective humoral and cell-mediated immune responses in several animal models. When compared with conventional vaccines, however, DNA vaccines often induce lower antibody titers. We have now found that formulation of a DNA vaccine encoding hepatitis B surface antigen with calcium- or aluminum phosphate adjuvants can increase antibody titers by 10-100-fold and decrease the immunogenic dose of DNA by 10-fold. Furthermore, boosting an HBs protein-primed response with the adjuvanted DNA vaccine resulted in a dramatic increase in the HBs-specific IgG2a response reflecting a shift towards a TH1 response. The mechanism by which aluminum phosphate exerts its adjuvant effect is not through increased expression of HBsAg in vivo; rather, the adjuvant appears to increase the number and affinity of HBs peptide antigen-specific IFN-gamma and IL-2 secreting T cells.Entities:
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Year: 2000 PMID： 10649624 DOI： 10.1016/s0264-410x(99)00391-6
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641