Literature DB >> 10648923

Generation of a large complex antibody library from multiple donors.

M Little1, M Welschof, M Braunagel, I Hermes, C Christ, A Keller, P Rohrbach, T Kürschner, S Schmidt, C Kleist, P Terness.   

Abstract

We have generated a large complex library of single chain antibodies based on four individual libraries from each of 50 donors. DNA coding for the heavy and light chain variable domains of the IgM and IgG repertoires was amplified by PCR using two different sets of primers. Each individual library was composed of approximately 1-5x10(7) independent clones giving a final combined library of 4x10(9) members. Screening this library by phage display of single chain antibodies with small haptens, peptides and proteins yielded specific antibodies for each class of antigen.

Mesh：

Substances：

Year:  1999        PMID： 10648923     DOI： 10.1016/s0022-1759(99)00164-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  9 in total

Review 1.  Recombinant antibodies for the diagnosis and treatment of cancer.

Authors:  Jürgen Krauss
Journal:  Mol Biotechnol       Date:  2003-09       Impact factor: 2.695

2.  The library of human miniantibodies in the phage display format: designing and testing.

Authors:  A B Ulitin; M V Kapralova; A G Laman; A O Shepelyakovskaya; E V Bulgakova; K K Fursova; S G Abbasova; S K Volkov; F A Brovko; V A Nesmeyanov
Journal:  Dokl Biochem Biophys       Date:  2005 Nov-Dec       Impact factor: 0.788

Review 3.  Peptide-displaying phage technology in glycobiology.

Authors:  Michiko N Fukuda
Journal:  Glycobiology       Date:  2011-09-19       Impact factor: 4.313

4.  Highly Specific and Effective Targeting of EGFRvIII-Positive Tumors with TandAb Antibodies.

Authors:  Kristina Ellwanger; Uwe Reusch; Ivica Fucek; Stefan Knackmuss; Michael Weichel; Thorsten Gantke; Vera Molkenthin; Eugene A Zhukovsky; Michael Tesar; Martin Treder
Journal:  Front Oncol       Date:  2017-05-19       Impact factor: 6.244

5.  Drug-like antibodies with high affinity, diversity and developability directly from next-generation antibody libraries.

Authors:  Andre Azevedo Reis Teixeira; Michael Frank Erasmus; Sara D'Angelo; Leslie Naranjo; Fortunato Ferrara; Camila Leal-Lopes; Oliver Durrant; Cecile Galmiche; Aleardo Morelli; Anthony Scott-Tucker; Andrew Raymon Morton Bradbury
Journal:  MAbs       Date:  2021 Jan-Dec       Impact factor: 5.857

6.  Generation and utility of a single-chain fragment variable monoclonal antibody platform against a baculovirus expressed recombinant receptor binding domain of SARS-CoV-2 spike protein.

Authors:  Reda Salem; Alaa A El-Kholy; Fatma R Waly; Dalia Ayman; Aya Sakr; Mai Hussein
Journal:  Mol Immunol       Date:  2021-12-10       Impact factor: 4.407

7.  A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C.

Authors:  Rajesh Kumar; Raiees Andrabi; Ashutosh Tiwari; Somi Sankaran Prakash; Naveet Wig; Durgashree Dutta; Anurag Sankhyan; Lubina Khan; Subrata Sinha; Kalpana Luthra
Journal:  BMC Biotechnol       Date:  2012-11-15       Impact factor: 2.563

8.  A compact phage display human scFv library for selection of antibodies to a wide variety of antigens.

Authors:  Potjamas Pansri; Nanthnit Jaruseranee; Kuntalee Rangnoi; Peter Kristensen; Montarop Yamabhai
Journal:  BMC Biotechnol       Date:  2009-01-29       Impact factor: 2.563

9.  Bactericidal fully human single-chain fragment variable antibodies protect mice against methicillin-resistant Staphylococcus aureus bacteraemia.

Authors:  Behnoush Soltanmohammadi; Somayeh Piri-Gavgani; Eilnaz Basardeh; Mostafa Ghanei; Masoumeh Azizi; Zabihollah Khaksar; Zahra Sharifzadeh; Farzad Badmasti; Mahdieh Soezi; Abolfazl Fateh; Parisa Azimi; Seyed Davar Siadat; Fahimeh Shooraj; Saeid Bouzari; Mir Davood Omrani; Fatemeh Rahimi-Jamnani
Journal:  Clin Transl Immunology       Date:  2021-06-29
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.