BACKGROUND & AIMS: The immune response of gastric T cells during acute Helicobacter pylori infection has not been previously characterized. The aim of this study was to delineate the phenotypic and functional responses of gastric T cells during acute H. pylori infection of rhesus macaques. METHODS: Four monkeys were experimentally infected with H. pylori. Gastric biopsy specimens and peripheral blood samples were obtained 1 and 12 weeks after inoculation. Samples from 3 animals uninfected with H. pylori served as controls. The immunophenotypic changes and functional potential of CD4(+) and CD8(+) T cells in gastric mucosa and peripheral blood to produce cytokines (interleukin [IL]-2, IL-4, IL-13, interferon [IFN]-gamma, MIP-1beta, and tumor necrosis factor [TNF]-alpha) were determined at a single cell level using flow cytometry. RESULTS: An increase in CD4(+) T cells occurred in the gastric mucosa during acute H. pylori infection as early as 1 week after infection. Acute infection was characterized by a predominantly T helper (Th)1 (IL-2 and IFN-gamma) and proinflammatory (TNF-alpha and MIP-1beta) type of cytokine response and the absence of a Th2 type of response. CONCLUSIONS: A predominant Th1 type response was induced early during acute H. pylori infection and may contribute to the development of gastric disease.
BACKGROUND & AIMS: The immune response of gastric T cells during acute Helicobacter pylori infection has not been previously characterized. The aim of this study was to delineate the phenotypic and functional responses of gastric T cells during acute H. pyloriinfection of rhesus macaques. METHODS: Four monkeys were experimentally infected with H. pylori. Gastric biopsy specimens and peripheral blood samples were obtained 1 and 12 weeks after inoculation. Samples from 3 animals uninfected with H. pylori served as controls. The immunophenotypic changes and functional potential of CD4(+) and CD8(+) T cells in gastric mucosa and peripheral blood to produce cytokines (interleukin [IL]-2, IL-4, IL-13, interferon [IFN]-gamma, MIP-1beta, and tumor necrosis factor [TNF]-alpha) were determined at a single cell level using flow cytometry. RESULTS: An increase in CD4(+) T cells occurred in the gastric mucosa during acute H. pyloriinfection as early as 1 week after infection. Acute infection was characterized by a predominantly T helper (Th)1 (IL-2 and IFN-gamma) and proinflammatory (TNF-alpha and MIP-1beta) type of cytokine response and the absence of a Th2 type of response. CONCLUSIONS: A predominant Th1 type response was induced early during acute H. pyloriinfection and may contribute to the development of gastric disease.
Authors: Michael A Schumacher; Jessica M Donnelly; Amy C Engevik; Chang Xiao; Li Yang; Susan Kenny; Andrea Varro; Frédéric Hollande; Linda C Samuelson; Yana Zavros Journal: Gastroenterology Date: 2012-01-25 Impact factor: 22.682
Authors: M Kraft; S Riedel; C Maaser; T Kucharzik; A Steinbuechel; W Domschke; N Luegering Journal: Clin Exp Immunol Date: 2001-12 Impact factor: 4.330
Authors: Carolina Serrano; Shelton W Wright; Diane Bimczok; Carrie L Shaffer; Timothy L Cover; Alejandro Venegas; Maria G Salazar; Lesley E Smythies; Paul R Harris; Phillip D Smith Journal: Mucosal Immunol Date: 2013-01-09 Impact factor: 7.313
Authors: Reinhard K Straubinger; Andrea Greiter; Sean P McDonough; Alexander Gerold; Eugenio Scanziani; Sabina Soldati; Daiva Dailidiene; Giedrius Dailide; Douglas E Berg; Kenneth W Simpson Journal: Infect Immun Date: 2003-05 Impact factor: 3.441
Authors: Ellen J Beswick; David A Bland; Giovanni Suarez; Carlos A Barrera; Xuejung Fan; Victor E Reyes Journal: Infect Immun Date: 2005-05 Impact factor: 3.441
Authors: Yana Zavros; Sivaprakash Rathinavelu; John Y Kao; Andrea Todisco; John Del Valle; Joel V Weinstock; Malcolm J Low; Juanita L Merchant Journal: Proc Natl Acad Sci U S A Date: 2003-10-10 Impact factor: 11.205