Literature DB >> 10645984

Quantitative direct probe method for the detection of parvovirus B19.

H Boggino1, D A Payne.   

Abstract

Parvovirus B19 infection is associated with anemia and spontaneous abortions. While many qualitative assays are available, a few molecular-based quantitative methods have been described. This study reports the development and optimization of a quantitative direct-probe method for the detection of Parvovirus B19 DNA. Different concentrations of RNA probes were used to identify the optimal conditions for hybridizing to the target DNA. Detection of DNA was linear between concentrations of 2 ng/ml to 200 pg/ml. Because this method requires no enzymatic amplification, it is not susceptible to amplifier contamination or enzymatic inhibitors, and it can be applied to serum samples or paraffin-embedded tissue. Copyright 2000 Wiley-Liss, Inc.

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Year:  2000        PMID： 10645984      PMCID： PMC6807923          DOI： 10.1002/(sici)1098-2825(2000)14:1<38::aid-jcla8>3.0.co;2-n

Source DB:  PubMed          Journal:  J Clin Lab Anal        ISSN： 0887-8013            Impact factor:   2.352


  21 in total

1.  Detection of B19 parvovirus in human fetal tissues by in situ hybridisation.

Authors:  J P Nascimento; N F Hallam; J Mori; A M Field; J P Clewley; K E Brown; B J Cohen
Journal:  J Med Virol       Date:  1991-02       Impact factor: 2.327

2.  Parvovirus associated aplastic crisis in homozygous sickle cell disease.

Authors:  A R Goldstein; M J Anderson; G R Serjeant
Journal:  Arch Dis Child       Date:  1987-06       Impact factor: 3.791

Review 3.  Parvoviruses as agents of human disease.

Authors:  M J Anderson
Journal:  Prog Med Virol       Date:  1987

4.  Automated detection of digoxigenin-labelled B19 parvovirus amplicons by a capture hybridization assay.

Authors:  M Zerbini; D Gibellini; M Musiani; S Venturoli; G Gallinella; G Gentilomi
Journal:  J Virol Methods       Date:  1995-09       Impact factor: 2.014

5.  Evaluation of strand-specific RNA probes visualized by colorimetric and chemiluminescent reactions for the detection of B19 parvovirus DNA.

Authors:  M Zerbini; M Musiani; D Gibellini; G Gentilomi; S Venturoli; G Gallinella; M La Placa
Journal:  J Virol Methods       Date:  1993-12-15       Impact factor: 2.014

6.  Human parvovirus infection in pregnancy and hydrops fetalis.

Authors:  A Anand; E S Gray; T Brown; J P Clewley; B J Cohen
Journal:  N Engl J Med       Date:  1987-01-22       Impact factor: 91.245

7.  Human parvovirus-associated red cell aplasia in the absence of underlying hemolytic anemia.

Authors:  D K Van Horn; P P Mortimer; N Young; G R Hanson
Journal:  Am J Pediatr Hematol Oncol       Date:  1986

8.  Rapid detection of human parvovirus B19 DNA by dot-hybridization and the polymerase chain reaction.

Authors:  M M Salimans; S Holsappel; F M van de Rijke; N M Jiwa; A K Raap; H T Weiland
Journal:  J Virol Methods       Date:  1989-01       Impact factor: 2.014

Review 9.  Analysis of parvovirus infections using strand-specific hybridization probes.

Authors:  M E Bloom; S Alexandersen; S Mori; J B Wolfinbarger
Journal:  Virus Res       Date:  1989-09       Impact factor: 3.303

10.  Non-isotopic in situ hybridisation and immunophenotyping of infected cells in the investigation of human fetal parvovirus infection.

Authors:  A L Morey; H J Porter; J W Keeling; K A Fleming
Journal:  J Clin Pathol       Date:  1992-08       Impact factor: 3.411
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