The role of local angiotensins and prostaglandins in the control of anion secretion by the rat epididymis.

Secretion of electrolytes and water by the epididymal epithelium is important in the formation of an optimal fluid environment for sperm maturation and storage. Recently, evidence has been obtained that anion/fluid secretion by the epididymis is subject to control by local humoral factors, among which the angiotensins play a significant role. This assertion is based on the morphological localization of various components of a local renin-angiotensin system (RAS) in the rat epididymis and the functional studies of angiotensins and their antagonists on anion secretion in cultured rat epididymal epithelia. More recent study has indicated that the effects of angiotensin II and other vasoactive peptides on anion secretion are mediated through an increase in prostaglandin formation. The pathway of synthesis involves the PLA2-coupled receptor mediated breakdown of membrane phospholipids to arachidonic acid followed by conversion of arachidonic acid into the prostanoids by cyclooxygenases and other enzymes. The newly formed PGE2 then diffuses out of the cells and acts on the EP2/4 receptors on the same or adjacent cells to increase intracellular cAMP. Accordingly, the pathways of activation by the paracrine factors all converge on the cystic fibrosis transmembrane conductance regulator (CFTR) as the final common effector in secretion. Studies on the biochemical pathways of paracrine control of fluid secretion may provide insight into the causes of epididymal irregularities in some forms of male infertility.