| Literature DB >> 10644889 |
R H Shyu1, M F Shaio, S S Tang, H F Shyu, C F Lee, M H Tsai, J E Smith, H H Huang, J J Wey, J L Huang, H H Chang.
Abstract
Botulinum neurotoxin (BoNT) is one of the most toxic substances known to produce severe neuromuscular paralysis. The currently used vaccine is prepared mainly from biohazardous toxins. Thus, we studied an alternative method and demonstrated that DNA immunization provided sufficient protection against botulism in a murine model. A plasmid of pBoNT/A-Hc, which encodes the fragment C gene of type A botulinum neurotoxin, was constructed and fused with an Igkappa leader sequence under the control of a human cytomegalovirus promoter. After 10 cycles of DNA inoculation with this plasmid, mice survived lethal doses of type A botulinum neurotoxin challenges. Immunized mice also elicited cross-protection to the challenges of type E botulinum neurotoxin. This is the first study demonstrating the potential use of DNA vaccination for botulinum neurotoxins. Copyright 2000 National Science Council, ROC and S. Karger AG, BaselEntities:
Mesh：
Substances：
Year: 2000 PMID： 10644889 DOI： 10.1007/bf02255918
Source DB: PubMed Journal: J Biomed Sci ISSN： 1021-7770 Impact factor: 8.410