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Literature DB >> 1064141

Isolation and perfusion of the porcine pancreas.

S L Jensen, C Kühl, O V Nielsen, J J Holst.

Abstract

The isolation of a porcine pancreas preparation without the attached duodenum is described. The preparation is suitable for study of the secretion of insulin and pancreatic glucagon. The surgical procedure by which the pancreas is removed from tha animal is decribed as well as the perfusion system. The effect of various concentrations of glucose (63, 90, 135 and 180 mg per 100 ml) in the perfusion medium on the secretion of insulin and pancreatic glucagon was investigated in order to observe the performance characteristics of this preparation. Increasing concentrations of glucose caused a significantly greater insulin release, whereas the glucagon response was significantly reduced.

Entities: Chemical Disease Gene

Mesh：

Substances：
Insulin
Glucagon
Glucose

Year: 1976 PMID： 1064141

Source DB: PubMed Journal: Scand J Gastroenterol Suppl ISSN： 0085-5928

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Cited

7 in total

1. Reduction of beta cell mass: partial insulin secretory compensation from the residual beta cell population in the nicotinamide-streptozotocin Göttingen minipig after oral glucose in vivo and in the perfused pancreas.

Authors: M O Larsen; B Rolin; C F Gotfredsen; R D Carr; J J Holst
Journal: Diabetologia Date: 2004-11-24 Impact factor: 10.122

2. Pig pancreas anatomy: implications for pancreas procurement, preservation, and islet isolation.

Authors: Joana Ferrer; William E Scott; Bradley P Weegman; Thomas M Suszynski; David E R Sutherland; Bernhard J Hering; Klearchos K Papas
Journal: Transplantation Date: 2008-12-15 Impact factor: 4.939

3. Functional and morphological differentiation of fetal porcine islet-like cell clusters after transplantation into nude mice.

Authors: O Korsgren; L Jansson; D Eizirik; A Andersson
Journal: Diabetologia Date: 1991-06 Impact factor: 10.122

Isolation and perfusion of the porcine pancreas.

1. Reduction of beta cell mass: partial insulin secretory compensation from the residual beta cell population in the nicotinamide-streptozotocin Göttingen minipig after oral glucose in vivo and in the perfused pancreas.

2. Pig pancreas anatomy: implications for pancreas procurement, preservation, and islet isolation.

3. Functional and morphological differentiation of fetal porcine islet-like cell clusters after transplantation into nude mice.

4. Vagal, cholinergic regulation of pancreatic polypeptide secretion.

5. Secretin release from the isolated, vascularly perfused pig duodenum.

6. Porcine pancreastatin has no effect on endocrine secretion from the pig pancreas.

Review 7. Pancreatic duct secretion: experimental methods, ion transport mechanisms and regulation.