I A Scordi1, M Nassiri, A J Hanly, V Vincek. 1. Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, FL 33101, USA.
Abstract
BACKGROUND: UVB radiation, the major cause of skin cancer, causes apoptosis in the skin, characterized by the appearance of 'sunburn cells'. Interleukin 11 (IL-11) has been shown to protect cells in the gastrointestinal system from chemotherapy and radiation-induced death. OBJECTIVE: To determine whether exogenous IL-11 has any effect on the UVB-induced apoptosis of cutaneous tissues. METHODS: Mice were injected with IL-11 or vehicle control prior to irradiation, and TUNEL assays were performed on paraffin-embedded tissue sections at various times after irradiation. RESULTS: Exogenous IL-11 protects epidermal keratinocytes, epithelial cells of the hair follicles inflammatory cells and fibroblasts in the dermis from UVB-induced apoptotic death. CONCLUSION: These findings suggest that IL-11 may play a role in the general homeostasis of the skin. Copyright 2000 S. Karger AG, Basel
BACKGROUND: UVB radiation, the major cause of skin cancer, causes apoptosis in the skin, characterized by the appearance of 'sunburn cells'. Interleukin 11 (IL-11) has been shown to protect cells in the gastrointestinal system from chemotherapy and radiation-induced death. OBJECTIVE: To determine whether exogenous IL-11 has any effect on the UVB-induced apoptosis of cutaneous tissues. METHODS:Mice were injected with IL-11 or vehicle control prior to irradiation, and TUNEL assays were performed on paraffin-embedded tissue sections at various times after irradiation. RESULTS: Exogenous IL-11 protects epidermal keratinocytes, epithelial cells of the hair follicles inflammatory cells and fibroblasts in the dermis from UVB-induced apoptotic death. CONCLUSION: These findings suggest that IL-11 may play a role in the general homeostasis of the skin. Copyright 2000 S. Karger AG, Basel