Literature DB >> 10639451

Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis.

Y Xu1, K V Singh, X Qin, B E Murray, G M Weinstock.   

Abstract

Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4, encoding a putative glycosyl transferase, and orfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.

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Year:  2000        PMID： 10639451      PMCID： PMC97210          DOI： 10.1128/IAI.68.2.815-823.2000

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  24 in total

1.  Enterococcus faecalis antigens in human infections.

Authors:  Y Xu; L Jiang; B E Murray; G M Weinstock
Journal:  Infect Immun       Date:  1997-10       Impact factor: 3.441

Review 2.  Molecular biology of the capsular genes of Streptococcus pneumoniae.

Authors:  E García; R López
Journal:  FEMS Microbiol Lett       Date:  1997-04-01       Impact factor: 2.742

Review 3.  Regulation of capsule synthesis includes interactions of the RcsC/RcsB regulatory pair.

Authors:  V Stout
Journal:  Res Microbiol       Date:  1994 Jun-Aug       Impact factor: 3.992

4.  Pseudomonas aeruginosa in cystic fibrosis: role of mucC in the regulation of alginate production and stress sensitivity.

Authors:  J C Boucher; M J Schurr; H Yu; D W Rowen; V Deretic
Journal:  Microbiology (Reading)       Date:  1997-11       Impact factor: 2.777

5.  A cluster of genes involved in polysaccharide biosynthesis from Enterococcus faecalis OG1RF.

Authors:  Y Xu; B E Murray; G M Weinstock
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

6.  Effect of disruption of a gene encoding an autolysin of Enterococcus faecalis OG1RF.

Authors:  X Qin; K V Singh; Y Xu; G M Weinstock; B E Murray
Journal:  Antimicrob Agents Chemother       Date:  1998-11       Impact factor: 5.191

7.  Capsular polysaccharide types 5 and 8 of Staphylococcus aureus bind specifically to human epithelial (KB) cells, endothelial cells, and monocytes and induce release of cytokines.

Authors:  M Soell; M Diab; G Haan-Archipoff; A Beretz; C Herbelin; B Poutrel; J P Klein
Journal:  Infect Immun       Date:  1995-04       Impact factor: 3.441

8.  Generation and testing of mutants of Enterococcus faecalis in a mouse peritonitis model.

Authors:  K V Singh; X Qin; G M Weinstock; B E Murray
Journal:  J Infect Dis       Date:  1998-11       Impact factor: 5.226

9.  Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA.

Authors:  J C Boucher; J Martinez-Salazar; M J Schurr; M H Mudd; H Yu; V Deretic
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490

10.  Mucoid encapsulated Enterococcus faecalis: an emerging morphotype isolated from patients with urinary tract infections.

Authors:  E J Bottone; L Patel; P Patel; T Robin
Journal:  Diagn Microbiol Infect Dis       Date:  1998-07       Impact factor: 2.803
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  56 in total

Review 1.  Relationships between enterococcal virulence and antimicrobial resistance.

Authors:  L M Mundy; D F Sahm; M Gilmore
Journal:  Clin Microbiol Rev       Date:  2000-10       Impact factor: 26.132

Review 2.  The rise of the Enterococcus: beyond vancomycin resistance.

Authors:  Cesar A Arias; Barbara E Murray
Journal:  Nat Rev Microbiol       Date:  2012-03-16       Impact factor: 60.633

Review 3.  Pathogenicity of Enterococci.

Authors:  Elizabeth Fiore; Daria Van Tyne; Michael S Gilmore
Journal:  Microbiol Spectr       Date:  2019-07

4.  Naturally acquired antibodies against four Enterococcus faecalis capsular polysaccharides in healthy human sera.

Authors:  Markus Hufnagel; Andrea Kropec; Christian Theilacker; Johannes Huebner
Journal:  Clin Diagn Lab Immunol       Date:  2005-08

5.  Secondary cell wall polymers of Enterococcus faecalis are critical for resistance to complement activation via mannose-binding lectin.

Authors:  Stefan Geiss-Liebisch; Suzan H M Rooijakkers; Agnieszka Beczala; Patricia Sanchez-Carballo; Karolina Kruszynska; Christian Repp; Tuerkan Sakinc; Evgeny Vinogradov; Otto Holst; Johannes Huebner; Christian Theilacker
Journal:  J Biol Chem       Date:  2012-08-20       Impact factor: 5.157

6.  An Enterococcus faecium secreted antigen, SagA, exhibits broad-spectrum binding to extracellular matrix proteins and appears essential for E. faecium growth.

Authors:  Fang Teng; Magdalena Kawalec; George M Weinstock; Waleria Hryniewicz; Barbara E Murray
Journal:  Infect Immun       Date:  2003-09       Impact factor: 3.441

7.  Molecular analysis of the Enterococcus faecalis serotype 2 polysaccharide determinant.

Authors:  Lynn E Hancock; Brett D Shepard; Michael S Gilmore
Journal:  J Bacteriol       Date:  2003-08       Impact factor: 3.490

8.  Comparative genomic analysis of pathogenic and probiotic Enterococcus faecalis isolates, and their transcriptional responses to growth in human urine.

Authors:  Heidi C Vebø; Margrete Solheim; Lars Snipen; Ingolf F Nes; Dag A Brede
Journal:  PLoS One       Date:  2010-08-31       Impact factor: 3.240

9.  The transcriptome of the nosocomial pathogen Enterococcus faecalis V583 reveals adaptive responses to growth in blood.

Authors:  Heidi C Vebø; Lars Snipen; Ingolf F Nes; Dag A Brede
Journal:  PLoS One       Date:  2009-11-04       Impact factor: 3.240

10.  Frequency of ace, epa and elrA Genes in Clinical and Environmental Strains of Enterococcus faecalis.

Authors:  Monika Eliza Lysakowska; Andrzej Denys; Monika Sienkiewicz
Journal:  Indian J Microbiol       Date:  2012-06-22       Impact factor: 2.461
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