Phenotyping of porcine apolipoprotein E using isoelectric focusing and localization of the APOE gene within the halothane-susceptibility linkage group.

A method has been developed for the phenotyping of pig apolipoprotein E. It was based on isoelectric focusing of sialidase-treated and delipidated blood plasma followed by immunoblotting. As the first antibody the anti-human apolipoprotein E antibody was used. The genetic polymorphism of pig apolipoprotein E appeared to be controlled by three alleles at one locus. A two-point linkage analysis was performed between certain loci belonging to the halothane linkage group and the APOE locus on material comprising 9 Zlotnicka Spotted boars, 30 Polish Large White sows, and their 160 offspring. A tight linkage between the A1BG and the APOE loci was documented (theta = 0.038).