| Literature DB >> 10616926 |
T Tanaka1, X Xuan, H Ikadai, I Igarashi, H Nagasawa, K Fujisaki, T Mikami, N Suzuki.
Abstract
In this study, the gene encoding Babesia equi merozoite antigen-2 was inserted into a baculovirus transfer vector, and a recombinant virus expressing B. equi merozoite antigen-2 was isolated. Two B. equi merozoite antigen-2-related recombinant baculovirus-expressed peptides of 25 and 30 kDa were detected with a murine anti serum against B. equi merozoite antigen-2; these corresponded to the native B. equi merozoite antigen-2 and were secreted into the supernatants of insect cell cultures. Recombinant B. equi merozoite antigen-2 was not effected by tunicamycin treatment, indicating that B. equi merozoite antigen-2 was not glycosylated. The potential of recombinant B. equi merozoite antigen-2 for use in the ELISA was evaluated by measuring the antibody response to B. equi merozoite antigen-2 in horses experimentally infected with B. equi.Entities:
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Year: 1999 PMID： 10616926 DOI： 10.1016/s0020-7519(99)00146-0
Source DB: PubMed Journal: Int J Parasitol ISSN： 0020-7519 Impact factor: 3.981