BACKGROUND: The hemagglutination inhibition (HI) test has been one of the standards, with the IgM antibody capture ELISA (MAC-ELISA), for the diagnosis of dengue virus infections. The spread of dengue throughout the world and the increasing number of cases to be tested makes an ELISA-format test for IgG antibodies to replace the HI test highly desirable. OBJECTIVES: Evaluate the use of the IgG-ELISA as a substitute for the HI test in dengue diagnosis. STUDY DESIGN: Paired serum samples defined as being from primary or secondary dengue virus infections by HI, were tested by an ELISA that detects IgG antibodies. The correlations of titers and serologic interpretations between these two tests were examined. RESULTS: The IgG-ELISA showed a low correlation with the HI in primary infections, and a higher correlation in secondary infections because of the influence of IgM antibodies in the HI test. Nevertheless, IgG ELISA titers could be reliably associated with primary or secondary infections when analyzed by days after onset of symptoms, and can be used to characterize the immune response after flavivirus infections. CONCLUSION: The combination of the IgM and IgG ELISAs may be used to serologically diagnose dengue virus infections, since the IgG ELISA can substitute for the HI test in characterizing the immune response to dengue virus infections.
BACKGROUND: The hemagglutination inhibition (HI) test has been one of the standards, with the IgM antibody capture ELISA (MAC-ELISA), for the diagnosis of dengue virus infections. The spread of dengue throughout the world and the increasing number of cases to be tested makes an ELISA-format test for IgG antibodies to replace the HI test highly desirable. OBJECTIVES: Evaluate the use of the IgG-ELISA as a substitute for the HI test in dengue diagnosis. STUDY DESIGN: Paired serum samples defined as being from primary or secondary dengue virus infections by HI, were tested by an ELISA that detects IgG antibodies. The correlations of titers and serologic interpretations between these two tests were examined. RESULTS: The IgG-ELISA showed a low correlation with the HI in primary infections, and a higher correlation in secondary infections because of the influence of IgM antibodies in the HI test. Nevertheless, IgG ELISA titers could be reliably associated with primary or secondary infections when analyzed by days after onset of symptoms, and can be used to characterize the immune response after flavivirus infections. CONCLUSION: The combination of the IgM and IgG ELISAs may be used to serologically diagnose dengue virus infections, since the IgG ELISA can substitute for the HI test in characterizing the immune response to dengue virus infections.
Authors: Tyler M Sharp; Parvathy Pillai; Elizabeth Hunsperger; Gilberto A Santiago; Teresa Anderson; Trina Vap; Jeremy Collinson; Bryan F Buss; Thomas J Safranek; Mark J Sotir; Emily S Jentes; Jorge L Munoz-Jordan; D Fermin Arguello Journal: Am J Trop Med Hyg Date: 2012-01 Impact factor: 2.345
Authors: Nieli Rodrigues da Costa Faria; Victor Edgar Fiestas Solorzano; Luiz José de Souza; Rita Maria Ribeiro Nogueira; Fernanda de Bruycker-Nogueira; Thaís Chouin-Carneiro; Jaqueline Bastos Santos Simões; Monique da Rocha Queiroz Lima; Luzia Maria de Oliveira Pinto; Claire Fernandes Kubelka; Manoela Heringer; Elzinandes Leal de Azeredo; Flavia Barreto Dos Santos Journal: Am J Trop Med Hyg Date: 2017-07 Impact factor: 2.345
Authors: Flavia B Dos Santos; Rita Maria R Nogueira; Monique R Q Lima; Thatiane S De Simone; Hermann G Schatzmayr; Elezer M B Lemes; Eva Harris; Marize P Miagostovich Journal: Clin Vaccine Immunol Date: 2007-03-28
Authors: E L Azeredo; L M De Oliveira-Pinto; S M Zagne; D I S Cerqueira; R M R Nogueira; C F Kubelka Journal: Clin Exp Immunol Date: 2006-02 Impact factor: 4.330
Authors: Monique da Rocha Queiroz Lima; Rita Maria Ribeiro Nogueira; Ana Maria Bispo de Filippis; Flavia Barreto dos Santos Journal: Clin Vaccine Immunol Date: 2011-04-27
Authors: Olga D Lorenzi; Christopher J Gregory; Luis Manuel Santiago; Héctor Acosta; Ivonne E Galarza; Elizabeth Hunsperger; Jorge Muñoz; Duy M Bui; M Steven Oberste; Silvia Peñaranda; Carlos García-Gubern; Kay M Tomashek Journal: Am J Trop Med Hyg Date: 2013-02-04 Impact factor: 2.345