Literature DB >> 10613885

Effects of amino acid substitutions at conserved and acidic residues within region 1.1 of Escherichia coli sigma(70).

C W Bowers1, A McCracken, A J Dombroski.   

Abstract

Amino acid substitutions in Escherichia coli sigma(70) were generated and characterized in an analysis of the role of region 1.1 in transcription initiation. Several acidic and conserved residues are tolerant of substitution. However, replacement of aspartic acid 61 with alanine results in inactivity caused by structural and functional thermolability.

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Year:  2000        PMID： 10613885      PMCID： PMC94262          DOI： 10.1128/JB.182.1.221-224.2000

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  25 in total

1.  Two amino acids in an RNA polymerase sigma factor involved in the recognition of adjacent base pairs in the -10 region of a cognate promoter.

Authors:  D Daniels; P Zuber; R Losick
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

2.  Altered promoter recognition by mutant forms of the sigma 70 subunit of Escherichia coli RNA polymerase.

Authors:  D A Siegele; J C Hu; W A Walter; C A Gross
Journal:  J Mol Biol       Date:  1989-04-20       Impact factor: 5.469

3.  Polypeptides containing highly conserved regions of transcription initiation factor sigma 70 exhibit specificity of binding to promoter DNA.

Authors:  A J Dombroski; W A Walter; M T Record; D A Siegele; C A Gross
Journal:  Cell       Date:  1992-08-07       Impact factor: 41.582

4.  Base-specific recognition of the nontemplate strand of promoter DNA by E. coli RNA polymerase.

Authors:  C W Roberts; J W Roberts
Journal:  Cell       Date:  1996-08-09       Impact factor: 41.582

5.  Mutation changing the specificity of an RNA polymerase sigma factor.

Authors:  P Zuber; J Healy; H L Carter; S Cutting; C P Moran; R Losick
Journal:  J Mol Biol       Date:  1989-04-20       Impact factor: 5.469

6.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel; J D Roberts; R A Zakour
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

7.  A promoter melting region in the primary sigma factor of Bacillus subtilis. Identification of functionally important aromatic amino acids.

Authors:  Y L Juang; J D Helmann
Journal:  J Mol Biol       Date:  1994-02-04       Impact factor: 5.469

8.  Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting.

Authors:  J C Rong; J D Helmann
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490

9.  Changes in conserved region 2 of Escherichia coli sigma 70 affecting promoter recognition.

Authors:  C Waldburger; T Gardella; R Wong; M M Susskind
Journal:  J Mol Biol       Date:  1990-09-20       Impact factor: 5.469

10.  Escherichia coli RNA polymerase binding and initiation of transcription on fragments of lambda rifd 18 DNA containing promoters for lambda genes and for rrnB, tufB, rplC,A, rplJ,L, and rpoB,C genes.

Authors:  W E Taylor; R R Burgess
Journal:  Gene       Date:  1979-08       Impact factor: 3.688
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  4 in total

1.  The role of region II in the RNA polymerase sigma factor sigma(N) (sigma(54)).

Authors:  E Southern; M Merrick
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

2.  Formation of intermediate transcription initiation complexes at pfliD and pflgM by sigma(28) RNA polymerase.

Authors:  J R Givens; C L McGovern; A J Dombroski
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

3.  In vitro properties of RpoS (sigma(S)) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted.

Authors:  J Gowrishankar; Kaneyoshi Yamamoto; P R Subbarayan; Akira Ishihama
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

4.  Distinct functions of regions 1.1 and 1.2 of RNA polymerase σ subunits from Escherichia coli and Thermus aquaticus in transcription initiation.

Authors:  Nataliya Miropolskaya; Artem Ignatov; Irina Bass; Ekaterina Zhilina; Danil Pupov; Andrey Kulbachinskiy
Journal:  J Biol Chem       Date:  2012-05-17       Impact factor: 5.157
  4 in total

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