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Literature DB >> 1060107

Functional level of rat liver tryptophan 2,3-dixoygenase messenger RNA during superinduction of enzyme with actinomycin D.

Abstract

Tryptophan 2,3-dioxygenase [EC 1.13.11.11; L-tryptophan:oxygen 2,3-oxidoreductase (decyclizing)] activity is induced by glucocorticoid hormones and superinduced by actinomycin D. Previous experiments had shown that hormonal induction of the enzyme activity is accompanied by parallel increases in tryptophan 2,3-dioxygenase mRNA level. In this study, we measured the tryptophan 2,3-dioxygenase mRNA levels during superinduction as well as hormonal induction, to determine whether superinduction of the enzyme activity is also mediated through changes in mRNA concentration. Tryptophan 2,3-dioxygenase mRNA was measured in a Krebs ascites cell-free protein synthesizing system supplemented with rabbit reticulocyte initiation factors. We found that during superinduction of the enzyme activity by actinomycin D, the mRNA level is identical to that of the actinomycin D-free controls. Our results do not, therefore, support the hypothesis that hormonal induction and/or superinduction of tryptophan 2,3-dioxygenase mRNA are regulated by a rapidly turning over repressor.

Entities: Chemical Disease Gene Species

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Year: 1975 PMID： 1060107 PMCID： PMC388705 DOI： 10.1073/pnas.72.11.4285

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

20 in total

1. Kinetics of steroid induction and deinduction of tyrosine aminotransferase synthesis in cultured hepatoma cells.

Authors: R A Steinberg; B B Levinson; G M Tomkins
Journal: Proc Natl Acad Sci U S A Date: 1975-06 Impact factor: 11.205

2. Superinduction of tyrosine aminotransferase by actinomycin D in rat hepatoma (HTC) cells.

Authors: E B Thompson; D K Granner; G M Tomkins
Journal: J Mol Biol Date: 1970-12-14 Impact factor: 5.469

3. Further evidence for posttranscriptional control of inducible tyrosine aminotransferase synthesis in cultured hepatoma cells.

Authors: G M Tomkins; B B Levinson; J D Baxter; L Dethlefsen
Journal: Nat New Biol Date: 1972-09-06

4. "Superinduction" of tyrosine transaminase in hepatoma cell cultures: differential inhibition of synthesis and turnover by actionomycin D.

Authors: J R Reel; F T Kenney
Journal: Proc Natl Acad Sci U S A Date: 1968-09 Impact factor: 11.205

5. Control of glutamine synthetase synthesis in the embryonic chick neural retina. A caution in the use of actinomycin D.

Authors: R J Schwartz
Journal: J Biol Chem Date: 1973-09-25 Impact factor: 5.157

6. Assessment of hormone action in cultured cells.

Authors: K L Lee; F T Kenney
Journal: Acta Endocrinol Suppl (Copenh) Date: 1971

7. Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.

Authors: G Schutz; M Beato; P Feigelson
Journal: Proc Natl Acad Sci U S A Date: 1973-04 Impact factor: 11.205

8. Further evidence against post-transcriptional control of inducible tyrosine aminotransferase synthesis in cultured hepatoma cells.

Authors: F T Kenney; K L Lee; C D Stiles; J E Fritz
Journal: Nat New Biol Date: 1973-12-19

9. Induction of glutamine synthetase in cultures of embryonic neural retina. Comments on the use of actinomycin D.

Authors: R E Jones; M H Moscona; A A Moscona
Journal: J Biol Chem Date: 1974-09-25 Impact factor: 5.157

10. Control of the mRNA for hepatic tryptophan oxygenase during hormonal and substrate induction.

Authors: G Schutz; L Killewich; G Chen; P Feigelson
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

5 in total

1. Interferon messenger RNA content of human fibroblasts during induction, shutoff, and superinduction of interferon production.

Authors: P B Sehgal; B Dobberstein; I Tamm
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

2. Thionein gene expression in Cd++-variants of the CHO cell: correlation of thionein synthesis rates with translatable mRNA levels during induction, deinduction, and superinduction.

Authors: M D Enger; L B Rall; C E Hildebrand
Journal: Nucleic Acids Res Date: 1979-09-11 Impact factor: 16.971