Literature DB >> 10596377

Fast isolation of recombinant baculovirus by antibody screening.

F Grosse1, A Manns.   

Abstract

We describe a rapid and efficient scheme for the isolation and purification of recombinant baculoviruses. The method is based on the detection of foreign proteins in cellular lysates of baculovirus-infected insect cells by antibody screening. The recombinant virus is purified by repeated serial dilutions. The method allows the identification and purification of recombinant viruses within 2 to 3 wk. This procedure selects for recombinant baculoviruses that highly overproduce the desired protein product.

Mesh：

Substances：

Year:  1999        PMID： 10596377     DOI： 10.1385/MB:12:2:217

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  4 in total

1.  Fast isolation of recombinant baculovirus by antibody screening.

Authors:  A Manns; F Grosse
Journal:  Biotechniques       Date:  1991-02       Impact factor: 1.993

2.  Screening recombinant baculovirus plaques in situ with antibody probes.

Authors:  J Capone
Journal:  Gene Anal Tech       Date:  1989 May-Jun

3.  Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization.

Authors:  M C Fung; K Y Chiu; T Weber; T W Chang; N T Chang
Journal:  J Virol Methods       Date:  1988-01       Impact factor: 2.014

4.  An efficient procedure for the isolation of recombinant baculovirus.

Authors:  J Pen; G W Welling; S Welling-Wester
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.