Literature DB >> 10596375

An antibody-lectin sandwich assay for quantifying protein glycoforms.

F T Lundy1, G B Wisdom.   

Abstract

We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein's glycoforms by simply substituting the antibody and lectin with specific alternatives.

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Year:  1999        PMID： 10596375     DOI： 10.1385/MB:12:2:203

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  7 in total

Review 1.  N-glycosylation of serum proteins in disease and its investigation using lectins.

Authors:  G A Turner
Journal:  Clin Chim Acta       Date:  1992-06-30       Impact factor: 3.786

2.  Alpha-fetoprotein antibody-lectin enzyme immunoassay to characterize sugar chains for the study of liver diseases.

Authors:  N Kinoshita; S Suzuki; Y Matsuda; N Taniguchi
Journal:  Clin Chim Acta       Date:  1989-02-15       Impact factor: 3.786

3.  Lectin-enzyme immunoassay of transferrin sialovariants using immobilized antitransferrin and enzyme-labeled galactose-binding lectin from Ricinus communis.

Authors:  J M Pekelharing; P Vissers; H A Peters; B Leijnse
Journal:  Anal Biochem       Date:  1987-09       Impact factor: 3.365

4.  Use of lectins for detection of electrophoretically separated glycoproteins transferred onto nitrocellulose sheets.

Authors:  W F Glass; R C Briggs; L S Hnilica
Journal:  Anal Biochem       Date:  1981-07-15       Impact factor: 3.365

5.  A sandwich method of enzymoimmunoassay. I. Application to rat and human alpha-fetoprotein.

Authors:  R Maiolini; R Masseyeff
Journal:  J Immunol Methods       Date:  1975-09       Impact factor: 2.303

6.  Modified (desialylated) low-density lipoprotein measured in serum by lectin-sorbent assay.

Authors:  V V Tertov; I A Sobenin; A N Orekhov
Journal:  Clin Chem       Date:  1995-07       Impact factor: 8.327

7.  A multiwell lectin-binding assay using lotus tetragonolobus for measuring different glycosylated forms of haptoglobin.

Authors:  S Thompson; R Stappenbeck; G A Turner
Journal:  Clin Chim Acta       Date:  1989-04-14       Impact factor: 3.786
  7 in total
  2 in total

1.  A novel core fucose-specific lectin from the mushroom Pholiota squarrosa.

Authors:  Yuka Kobayashi; Hiroaki Tateno; Hideo Dohra; Kenta Moriwaki; Eiji Miyoshi; Jun Hirabayashi; Hirokazu Kawagishi
Journal:  J Biol Chem       Date:  2012-08-07       Impact factor: 5.157

2.  A Quantitative Microtiter Assay for Sialylated Glycoform Analyses Using Lectin Complexes.

Authors:  Karunya Srinivasan; Sucharita Roy; Nathaniel Washburn; Sandra F Sipsey; Robin Meccariello; James W Meador; Leona E Ling; Anthony M Manning; Ganesh V Kaundinya
Journal:  J Biomol Screen       Date:  2015-04-07
  2 in total

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