OBJECTIVE: To find out the in vitro reaction of mesothelial cells and polymorphonuclear leucocytes (PMN) to incubation with seven commonly-used lavage solutions. DESIGN: Experimental study. SETTING: Laboratories, The Netherlands. MATERIAL: Cultured human peritoneal mesothelial cells and isolated PMN. INTERVENTION: Incubation of cells with clinically used lavage solutions (sodium chloride, Hartmann's solution, povidone-iodine, Dakin's solution, taurolidine, chlorhexidine, and hydrogen peroxide). MAIN OUTCOME MEASURES: Activation of monolayers of mesothelial cells and PMN measured by release of oxygen free radicals (chemiluminescence) and interleukin (IL)-8 concentrations and toxic effects measured by morphology, release of lactate dehydrogenase, failure of the restriction of the passage of inulin, and incorporation of propidium iodide. RESULTS: All solutions activated and killed mesothelial cells and PMN to some extent; the more concentrated the solution the greater the effect on these cells. CONCLUSION: Lavage solutions both poison and stimulate mesothelial cells and neutrophils, and some solutions are more potent than others.
OBJECTIVE: To find out the in vitro reaction of mesothelial cells and polymorphonuclear leucocytes (PMN) to incubation with seven commonly-used lavage solutions. DESIGN: Experimental study. SETTING: Laboratories, The Netherlands. MATERIAL: Cultured human peritoneal mesothelial cells and isolated PMN. INTERVENTION: Incubation of cells with clinically used lavage solutions (sodium chloride, Hartmann's solution, povidone-iodine, Dakin's solution, taurolidine, chlorhexidine, and hydrogen peroxide). MAIN OUTCOME MEASURES: Activation of monolayers of mesothelial cells and PMN measured by release of oxygen free radicals (chemiluminescence) and interleukin (IL)-8 concentrations and toxic effects measured by morphology, release of lactate dehydrogenase, failure of the restriction of the passage of inulin, and incorporation of propidium iodide. RESULTS: All solutions activated and killed mesothelial cells and PMN to some extent; the more concentrated the solution the greater the effect on these cells. CONCLUSION: Lavage solutions both poison and stimulate mesothelial cells and neutrophils, and some solutions are more potent than others.
Authors: Yannick Wouters; Gina R H Mennen; René H M Te Morsche; Hennie M J Roelofs; Geert J A Wanten Journal: In Vivo Date: 2022 Sep-Oct Impact factor: 2.406