Literature DB >> 10594350

A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines.

M Spagnuolo-Weaver1, R Fuerst, S T Campbell, B M Meehan, F McNeilly, B Adair, G Allan.   

Abstract

In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1alpha (IL1alpha) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and compare it to a standard PCR performed in a block-based thermocycler.

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Year:  1999        PMID： 10594350     DOI： 10.1016/s0022-1759(99)00114-3

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  6 in total

1.  The effect of orexin B on steroidogenic acute regulatory protein, P450 side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase gene expression, and progesterone and androstenedione secretion by the porcine uterus during early pregnancy and the estrous cycle.

Authors:  Katarzyna Kisielewska; Edyta Rytelewska; Marlena Gudelska; Marta Kiezun; Kamil Dobrzyn; Karol Szeszko; Kinga Bors; Joanna Wyrebek; Tadeusz Kaminski; Nina Smolinska
Journal:  J Anim Sci       Date:  2019-02-01       Impact factor: 3.159

2.  The influence of adiponectin on the transcriptomic profile of porcine luteal cells.

Authors:  Karol Szeszko; Nina Smolinska; Marta Kiezun; Kamil Dobrzyn; Anna Maleszka; Tadeusz Kaminski
Journal:  Funct Integr Genomics       Date:  2015-12-29       Impact factor: 3.410

3.  The In Vitro Effect of Prostaglandin E2 and F2α on the Chemerin System in the Porcine Endometrium during Gestation.

Authors:  Kamil Dobrzyn; Marta Kiezun; Ewa Zaobidna; Katarzyna Kisielewska; Edyta Rytelewska; Marlena Gudelska; Grzegorz Kopij; Kinga Bors; Karolina Szymanska; Barbara Kaminska; Tadeusz Kaminski; Nina Smolinska
Journal:  Int J Mol Sci       Date:  2020-07-23       Impact factor: 5.923

4.  The in vitro effect of progesterone on the orexin system in porcine uterine tissues during early pregnancy.

Authors:  Kamil Dobrzyn; Nina Smolinska; Marta Kiezun; Karol Szeszko; Edyta Rytelewska; Katarzyna Kisielewska; Marlena Gudelska; Tadeusz Kaminski
Journal:  Acta Vet Scand       Date:  2018-11-26       Impact factor: 1.695

5.  Chemerin Impact on Alternative mRNA Transcription in the Porcine Luteal Cells.

Authors:  Karol G Makowczenko; Jan P Jastrzebski; Lukasz Paukszto; Kamil Dobrzyn; Marta Kiezun; Nina Smolinska; Tadeusz Kaminski
Journal:  Cells       Date:  2022-02-17       Impact factor: 6.600

6.  Transcription Analysis of the Chemerin Impact on Gene Expression Profile in the Luteal Cells of Gilts.

Authors:  Karol G Makowczenko; Jan P Jastrzebski; Karol Szeszko; Nina Smolinska; Lukasz Paukszto; Kamil Dobrzyn; Marta Kiezun; Edyta Rytelewska; Barbara Kaminska; Tadeusz Kaminski
Journal:  Genes (Basel)       Date:  2020-06-12       Impact factor: 4.096
  6 in total

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