Literature DB >> 10593490

Characterization of SIV-specific CD4+ T-helper proliferative responses in macaques immunized with live-attenuated SIV.

M C Gauduin1, R L Glickman, S Ahmad, T Yilma, R P Johnson.   

Abstract

Analysis of immune responses generated by live-attenuated simian immunodeficiency virus (SIV) strains may provide clues to the mechanisms of protective immunity induced by this approach. We examined SIV-specific T-helper responses in macaques immunized with the live-attenuated SIV strains SIVmac239deltanef and SIVmac239delta3. Optimization of the concentration and duration of antigenic stimulation resulted in the detection of relatively strong SIV-specific proliferative responses, with peak stimulation indices of up to 84. SIV-specific proliferative responses were mediated by CD4+ T cells and were major histocompatibility (MHC) class II restricted. Limiting dilution analysis revealed SIV-specific T-helper precursor frequencies of up to 96 per 10(6) peripheral blood mononuclear cells (PBMC). Intracellular flow-cytometric analysis demonstrated the production of interleukin (IL)-2, interferon (IFN)-gamma, RANTES and macrophage inhibitory protein-1alpha (MIP-1alpha) by T lymphocytes from SIVmac239deltanef-vaccinated animals following SIV p55 stimulation. Induction of strong SIV-specific T-helper responses by live-attenuated SIV vaccines may play a role in their ability to induce protective immunity.

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Year:  1999        PMID： 10593490     DOI： 10.1111/j.1600-0684.1999.tb00274.x

Source DB:  PubMed          Journal:  J Med Primatol        ISSN： 0047-2565            Impact factor:   0.667


  10 in total

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2.  Soluble multi-trimeric TNF superfamily ligand adjuvants enhance immune responses to a HIV-1 Gag DNA vaccine.

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3.  Priming-boosting vaccination with recombinant Mycobacterium bovis bacillus Calmette-Guérin and a nonreplicating vaccinia virus recombinant leads to long-lasting and effective immunity.

Authors:  Yasushi Ami; Yasuyuki Izumi; Kazuhiro Matsuo; Kenji Someya; Masaru Kanekiyo; Shigeo Horibata; Naoto Yoshino; Koji Sakai; Katsuaki Shinohara; Sohkichi Matsumoto; Takeshi Yamada; Shudo Yamazaki; Naoki Yamamoto; Mitsuo Honda
Journal:  J Virol       Date:  2005-10       Impact factor: 5.103

4.  Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production.

Authors:  M C Gauduin; R L Glickman; S Ahmad; T Yilma; R P Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1999-11-23       Impact factor: 11.205

5.  Rev-independent simian immunodeficiency virus strains are nonpathogenic in neonatal macaques.

Authors:  Agneta S von Gegerfelt; Vladimir Liska; Pei-Lin Li; Harold M McClure; Kyoji Horie; Filomena Nappi; David C Montefiori; George N Pavlakis; Marta L Marthas; Ruth M Ruprecht; Barbara K Felber
Journal:  J Virol       Date:  2002-01       Impact factor: 5.103

6.  Flow cytometry based identification of simian immunodeficiency virus Env-specific B lymphocytes.

Authors:  Ismael Ben F Fofana; Arnaud D Colantonio; R Keith Reeves; Michelle A Connole; Jacqueline M Gillis; Laura R Hall; Shuji Sato; Craig R Audin; David T Evans; Hisatoshi Shida; R Paul Johnson; Welkin E Johnson
Journal:  J Immunol Methods       Date:  2011-06-13       Impact factor: 2.303

7.  In vivo attenuation of simian immunodeficiency virus by disruption of a tyrosine-dependent sorting signal in the envelope glycoprotein cytoplasmic tail.

Authors:  P N Fultz; P J Vance; M J Endres; B Tao; J D Dvorin; I C Davis; J D Lifson; D C Montefiori; M Marsh; M H Malim; J A Hoxie
Journal:  J Virol       Date:  2001-01       Impact factor: 5.103

8.  Simian-human immunodeficiency virus SHIV89.6-induced protection against intravaginal challenge with pathogenic SIVmac239 is independent of the route of immunization and is associated with a combination of cytotoxic T-lymphocyte and alpha interferon responses.

Authors:  Kristina Abel; Lara Compton; Tracy Rourke; David Montefiori; Ding Lu; Kristina Rothaeusler; Linda Fritts; Kristen Bost; Christopher J Miller
Journal:  J Virol       Date:  2003-03       Impact factor: 5.103

9.  Intravenous inoculation of replication-deficient recombinant vaccinia virus DIs expressing simian immunodeficiency virus gag controls highly pathogenic simian-human immunodeficiency virus in monkeys.

Authors:  Yasuyuki Izumi; Yasushi Ami; Kazuhiro Matsuo; Kenji Someya; Tetsutaro Sata; Naoki Yamamoto; Mitsuo Honda
Journal:  J Virol       Date:  2003-12       Impact factor: 5.103

10.  Immunization with single-cycle SIV significantly reduces viral loads after an intravenous challenge with SIV(mac)239.

Authors:  Bin Jia; Sharon K Ng; M Quinn DeGottardi; Michael Piatak; Eloísa Yuste; Angela Carville; Keith G Mansfield; Wenjun Li; Barbra A Richardson; Jeffrey D Lifson; David T Evans
Journal:  PLoS Pathog       Date:  2009-01-23       Impact factor: 6.823
  10 in total

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