Recombinational landscape across a 650-kb contig on the right arm of linkage group V in Neurospora crassa.

We have established a 650-kb walk spanning the sp to ure-1 interval on Neurospora crassa linkage group V. Loci covered by the walk are, from proximal to distal, sp; rec-2; ure-2; DNA polymerase delta; am; gul-1; ace-5; and ure-1. We have found extensive DNA polymorphism in this region and used this to examine the recombinational landscape. Crossovers are not evenly distributed over the region covered by the contig.