| Literature DB >> 10591649 |
T Preckel1, W P Fung-Leung, Z Cai, A Vitiello, L Salter-Cid, O Winqvist, T G Wolfe, M Von Herrath, A Angulo, P Ghazal, J D Lee, A M Fourie, Y Wu, J Pang, K Ngo, P A Peterson, K Früh, Y Yang.
Abstract
In vitro PA28 binds and activates proteasomes. It is shown here that mice with a disrupted PA28b gene lack PA28a and PA28b polypeptides, demonstrating that PA28 functions as a hetero-oligomer in vivo. Processing of antigenic epitopes derived from exogenous or endogenous antigens is altered in PA28-/- mice. Cytotoxic T lymphocyte responses are impaired, and assembly of immunoproteasomes is greatly inhibited in mice lacking PA28. These results show that PA28 is necessary for immunoproteasome assembly and is required for efficient antigen processing, thus demonstrating the importance of PA28-mediated proteasome function in immune responses.Entities:
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Year: 1999 PMID： 10591649 DOI： 10.1126/science.286.5447.2162
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728