| Literature DB >> 1059114 |
Abstract
Processing of rRNA was examined in resting and phytohemagglutinin-stimulated guinea pig lymphocytes. Synthesis of 1.7 (28S) and 0.7 (18S) X 10(6) dalton rRNA was more than 4-fold greater in phytohemagglutinin-stimulated than in resting cells. A 5- to 10-fold increase in flux of molecules through a 2.3 X 10(6) dalton RNA occurred without a concurrent change in the flux through a 2.6 X 10(6) dalton fraction in phytohemagglutinin-stimulated cells. In both resting and phytohemagglutinin-stimulated lymphocytes, the 2.3 X 10(6) dalton intermediate equilibrated with [3H]methyl label and pulse-chased prior to the 2.6 X 10(6) dalton RNA. The data indicate at least two processing paths in guinea pig lymphocytes; one proceeds to rRNA via a 2.3 X 10(6) dalton intermediate, and another proceeds via a 2.6 X 10(6) dalton RNA. The increase in rRNA synthesis in phytohemagglutinin-stimulated cells occurs primarily through that path containing the 2.3 X 10(6) dalton intermediate.Entities:
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Year: 1975 PMID： 1059114 PMCID： PMC432982 DOI： 10.1073/pnas.72.9.3315
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205