How I learned to love carrots: the role of the cytoskeleton in shaping plant cells.

The carrot cell suspension was originally used because it provided a model system for studying directional cell expansion - a key process in plant morphogenesis. Early immunofluorescence studies of plant microtubules, using these cells, provided hints that the cortical array of microtubules was dynamic and this was later confirmed by microinjection studies on plant epidermal cells. A nonfixation approach for detecting F-actin was then developed on these cells and showed that, unlike animal cells, actin filaments remained associated with the nucleus throughout division and could have a role in aligning the plane of cell division. Currently, we are using detergent-extracted carrot cytoskeletons for isolating microtubule-associated proteins (MAPs). I discuss how MAPs may be involved in the oriented deposition of cellulose in the cell wall. Copyright 1999 John Wiley & Sons, Inc.