PURPOSE: Our purpose was to investigate the effect of alpha- and beta-globulins contained in protein supplements on the development of preimplantation embryos. METHODS: Mouse one-cell embryos were cultured in medium supplemented with 4 mg/ml human serum albumin (HSA), 4 mg/ml HSA plus human globulins (0.2, 0.4, 0.8, and 1.6 mg/ml) that consisted predominantly of alpha- and beta-globulins, or 10% Plasmanate Cutter (PC). Blastocysts developed in media supplemented with these various protein sources were stained with Hoechst 33342 to determine the number of cells. RESULTS: Supplementation with 0.4 to 1.6 mg/ml globulins or PC significantly increased the rate of blastocyst development compared with that observed with the addition of HSA. Supplementation with globulins significantly increased the hatching rate in a dose-dependent manner. The number of cells in the blastocysts was significantly increased when the embryos were cultured with 0.8 mg/ml of the globulins or PC. CONCLUSIONS: The present observations suggest that alpha- and beta-globulins in protein supplements promote embryo development and hatching.
PURPOSE: Our purpose was to investigate the effect of alpha- and beta-globulins contained in protein supplements on the development of preimplantation embryos. METHODS:Mouse one-cell embryos were cultured in medium supplemented with 4 mg/ml humanserum albumin (HSA), 4 mg/ml HSA plus human globulins (0.2, 0.4, 0.8, and 1.6 mg/ml) that consisted predominantly of alpha- and beta-globulins, or 10% Plasmanate Cutter (PC). Blastocysts developed in media supplemented with these various protein sources were stained with Hoechst 33342 to determine the number of cells. RESULTS: Supplementation with 0.4 to 1.6 mg/ml globulins or PC significantly increased the rate of blastocyst development compared with that observed with the addition of HSA. Supplementation with globulins significantly increased the hatching rate in a dose-dependent manner. The number of cells in the blastocysts was significantly increased when the embryos were cultured with 0.8 mg/ml of the globulins or PC. CONCLUSIONS: The present observations suggest that alpha- and beta-globulins in protein supplements promote embryo development and hatching.
Authors: N N Desai; L A Sheean; D Martin; V Gindlesperger; C M Austin; H Lisbonna; B Peskin; J M Goldfarb Journal: J Assist Reprod Genet Date: 1996-01 Impact factor: 3.412
Authors: K E Tucker; B S Hurst; S Guadagnoli; C Dymecki; B Mendelsberg; C A Awoniyi; W D Schlaff Journal: J Assist Reprod Genet Date: 1996-01 Impact factor: 3.412
Authors: Y Onohara; T Harada; M Tanikawa; T Iwabe; H Yoshioka; F Taniguchi; M Mitsunari; T Tsudo; N Terakawa Journal: J Assist Reprod Genet Date: 1998-07 Impact factor: 3.412