| Literature DB >> 10574208 |
J Qiu1.
Abstract
HPLC analysis for organic acids in tobacco was optimized with the aid of statistical experimental design, a central composite face-centered design. In the design, only thirteen HPLC analyses were needed for identifying two optimal separation parameters. A Bio-Rad Aminex HPX-87H column was used for the analyses. An optimal separation for seven acids in tobacco was found at a temperature of 57 degrees C and a mobile phase of 0.032 N sulfuric acid solution, or at a temperature of 70 degrees C and a mobile phase of 0.024 N sulfuric acid solution, with a flow rate of 0.6 ml min(-1).Entities:
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Year: 1999 PMID： 10574208 DOI： 10.1016/s0021-9673(99)00886-9
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759