W Yu1, G Song, M Long. 1. Bioengineering College, Chongqing University.
Abstract
OBJECTIVE: To study the adhesive forces of HTC cells on different concentration of artificial basement membrane (collagen IV coated)and synchronous G1&S phase HTC cells on a certain concentration of artificial basement membrane. METHODS: The adhesive forces of HTC cells were investigated by micropipette aspiration technique. The synchronous G1 and S phase cells were achieved through thymine-2-desoryriboside and cochicine sequential blockage method and double thymine-2-desoryriboside blockage method respectively. RESULTS: HTC cells with 72.10% of G1 phase and 98.94% of S phase were achieved through thymine-2-desoryriboside and cochicine sequential blockage method and double thymine-2-desoryriboside blockage method respectively. The adhesive force of HTC cells on artificial basement membrane was in direct proportion to the concentration of collagen IV. G1 phase HTC cells had higher adhesive forces than S phase cells. CONCLUSION: These studies suggested that the increase of basement membrane might be conducive to the chemotactic motion and adhesion of tumor cells; G1 phase cells were more capable of adhering to and getting through basement membrane than S phase cells, and reflected the differences of receptors between G1 phase cells and S phase cells.
OBJECTIVE: To study the adhesive forces of HTC cells on different concentration of artificial basement membrane (collagen IV coated)and synchronous G1&S phase HTC cells on a certain concentration of artificial basement membrane. METHODS: The adhesive forces of HTC cells were investigated by micropipette aspiration technique. The synchronous G1 and S phase cells were achieved through thymine-2-desoryriboside and cochicine sequential blockage method and double thymine-2-desoryriboside blockage method respectively. RESULTS: HTC cells with 72.10% of G1 phase and 98.94% of S phase were achieved through thymine-2-desoryriboside and cochicine sequential blockage method and double thymine-2-desoryriboside blockage method respectively. The adhesive force of HTC cells on artificial basement membrane was in direct proportion to the concentration of collagen IV. G1 phase HTC cells had higher adhesive forces than S phase cells. CONCLUSION: These studies suggested that the increase of basement membrane might be conducive to the chemotactic motion and adhesion of tumor cells; G1 phase cells were more capable of adhering to and getting through basement membrane than S phase cells, and reflected the differences of receptors between G1 phase cells and S phase cells.