| Literature DB >> 10572188 |
J Zhang1, K O Voss, D F Shaw, K P Roos, D F Lewis, J Yan, R Jiang, H Ren, J Y Hou, Y Fang, X Puyang, H Ahmadzadeh, N J Dovichi.
Abstract
A five-capillary system has been developed for DNA sequencing and analysis. The post-column fluorescence detector is based on a sheath-flow cuvette. The instrument provides uniform and continuous illumination of the samples. The cuvette virtually eliminates cross-talk in the fluorescence signal between capillaries. Discrete single-photon counting avalanche photodiodes provide high efficiency light detection. The instrument has detection limits (3sigma) of 130 +/- 30 fluorescein molecules injected onto each capillary. Over 650 bases of sequence at 98.8% accuracy were generated in 100 min at 50 degrees C from M13mp18. Separation and detection of short tandem repeats proved efficient and accurate with the use of internal standards for direct comparison of migration times between capillaries.Entities:
Mesh：
Year: 1999 PMID： 10572188 PMCID： PMC148759 DOI： 10.1093/nar/27.24.e36
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971