| Literature DB >> 10572147 |
F Narberhaus1, C Urech, H Hennecke.
Abstract
The Bradyrhizobium japonicum ftsH gene was cloned by using a set of widely applicable degenerated oligonucleotides. Western blot experiments indicated that the FtsH protein was produced under standard growth conditions and that it was not heat inducible. Attempts to delete the ftsH gene in B. japonicum failed, suggesting a pivotal cellular function of this gene. The expression of B. japonicum ftsH in an ftsH-negative Escherichia coli strain significantly enhanced the fitness of this mutant and reduced the steady-state level of sigma(32).Entities:
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Year: 1999 PMID： 10572147 PMCID： PMC103706
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490