Literature DB >> 10571884

Technical Advance: A rapid method for detection of plant gene transcripts from single epidermal, mesophyll and companion cells of intact leaves.

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Abstract

A protocol for the detection of gene transcripts from single plant cells in living, undamaged plant tissue is described. Samples of leaf epidermal, mesophyll and companion cells were extracted by using glass microcapillaries and directly subjected to RT-PCR without any purification steps nor time consuming construction of cDNA libraries. The procedure is not restricted to surface cells or outer cell layers. Even cells from the central region of leaves could be harvested. For identification, companion cells were labelled by expression of the green fluorescent protein under control of a companion cell specific promoter. The described method is applicable to a wide range of plants and genes with different expression levels.

Year:  1999        PMID： 10571884     DOI： 10.1046/j.1365-313x.1999.00583.x

Source DB:  PubMed          Journal:  Plant J        ISSN： 0960-7412            Impact factor:   6.417


  16 in total

1.  Exploiting secondary growth in Arabidopsis. Construction of xylem and bark cDNA libraries and cloning of three xylem endopeptidases.

Authors:  C Zhao; B J Johnson; B Kositsup; E P Beers
Journal:  Plant Physiol       Date:  2000-07       Impact factor: 8.340

2.  Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize.

Authors:  Mikio Nakazono; Fang Qiu; Lisa A Borsuk; Patrick S Schnable
Journal:  Plant Cell       Date:  2003-03       Impact factor: 11.277

3.  Remote control - cell and organ communication within plants.

Authors:  Barbara Köhler; Bernd Mueller-Roeber
Journal:  New Phytol       Date:  2004-02       Impact factor: 10.151

4.  Rubisco small subunit, chlorophyll a/b-binding protein and sucrose:fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light.

Authors:  Chungui Lu; Olga A Koroleva; John F Farrar; Joe Gallagher; Chris J Pollock; A Deri Tomos
Journal:  Plant Physiol       Date:  2002-11       Impact factor: 8.340

5.  Genome-wide identification of genes expressed in Arabidopsis pistils specifically along the path of pollen tube growth.

Authors:  Chih-Wei Tung; Kathleen G Dwyer; Mikhail E Nasrallah; June B Nasrallah
Journal:  Plant Physiol       Date:  2005-05-13       Impact factor: 8.340

6.  Metabolic profiling of Arabidopsis thaliana epidermal cells.

Authors:  Berit Ebert; Daniela Zöller; Alexander Erban; Ines Fehrle; Jürgen Hartmann; Annette Niehl; Joachim Kopka; Joachim Fisahn
Journal:  J Exp Bot       Date:  2010-02-11       Impact factor: 6.992

7.  Tissue-specific transcriptome profiling of the citrus fruit epidermis and subepidermis using laser capture microdissection.

Authors:  Antonio J Matas; Javier Agustí; Francisco R Tadeo; Manuel Talón; Jocelyn K C Rose
Journal:  J Exp Bot       Date:  2010-06-02       Impact factor: 6.992

8.  Conserved cis-regulatory elements for DNA-binding-with-one-finger and homeo-domain-leucine-zipper transcription factors regulate companion cell-specific expression of the Arabidopsis thaliana SUCROSE TRANSPORTER 2 gene.

Authors:  Alexander Schneidereit; Astrid Imlau; Norbert Sauer
Journal:  Planta       Date:  2008-06-13       Impact factor: 4.116

9.  Quantitative expression analysis of selected transcription factors in pavement, basal and trichome cells of mature leaves from Arabidopsis thaliana.

Authors:  Martin Schliep; Berit Ebert; Ulrike Simon-Rosin; Daniela Zoeller; Joachim Fisahn
Journal:  Protoplasma       Date:  2010-01-26       Impact factor: 3.356

10.  Statistical evaluation of transcriptomic data generated using the Affymetrix one-cycle, two-cycle and IVT-Express RNA labelling protocols with the Arabidopsis ATH1 microarray.

Authors:  Tara J Holman; Michael H Wilson; Kim Kenobi; Ian L Dryden; T Charlie Hodgman; Andrew Ta Wood; Michael J Holdsworth
Journal:  Plant Methods       Date:  2010-03-15       Impact factor: 4.993
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