Literature DB >> 10569794

Constitutive and inducible expression of green fluorescent protein in Brucella suis.

S Köhler1, S Ouahrani-Bettache, M Layssac, J Teyssier, J P Liautard.   

Abstract

A gene fusion system based on plasmid pBBR1MCS and the expression of green fluorescent protein was developed for Brucella suis, allowing isolation of constitutive and inducible genes. Bacteria containing promoter fusions of chromosomal DNA to gfp were visualized by fluorescence microscopy and examined by flow cytometry. Twelve clones containing gene fragments induced inside J774 murine macrophages were isolated and further characterized.

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Year:  1999        PMID： 10569794      PMCID： PMC97086          DOI： 10.1128/IAI.67.12.6695-6697.1999

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  19 in total

1.  Basic local alignment search tool.

Authors:  S F Altschul; W Gish; W Miller; E W Myers; D J Lipman
Journal:  J Mol Biol       Date:  1990-10-05       Impact factor: 5.469

Review 2.  Interactions between professional phagocytes and Brucella spp.

Authors:  J P Liautard; A Gross; J Dornand; S Köhler
Journal:  Microbiologia       Date:  1996-06

3.  pBBR1MCS: a broad-host-range cloning vector.

Authors:  M E Kovach; R W Phillips; P H Elzer; R M Roop; K M Peterson
Journal:  Biotechniques       Date:  1994-05       Impact factor: 1.993

4.  Deletion of purE attenuates Brucella melitensis infection in mice.

Authors:  R M Crawford; L Van De Verg; L Yuan; T L Hadfield; R L Warren; E S Drazek; H H Houng; C Hammack; K Sasala; T Polsinelli; J Thompson; D L Hoover
Journal:  Infect Immun       Date:  1996-06       Impact factor: 3.441

5.  FACS-optimized mutants of the green fluorescent protein (GFP).

Authors:  B P Cormack; R H Valdivia; S Falkow
Journal:  Gene       Date:  1996       Impact factor: 3.688

6.  Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions.

Authors:  R H Valdivia; A E Hromockyj; D Monack; L Ramakrishnan; S Falkow
Journal:  Gene       Date:  1996       Impact factor: 3.688

7.  Early acidification of phagosomes containing Brucella suis is essential for intracellular survival in murine macrophages.

Authors:  F Porte; J P Liautard; S Köhler
Journal:  Infect Immun       Date:  1999-08       Impact factor: 3.441

8.  In vivo and in vitro stability of the broad-host-range cloning vector pBBR1MCS in six Brucella species.

Authors:  P H Elzer; M E Kovach; R W Phillips; G T Robertson; K M Peterson; R M Roop
Journal:  Plasmid       Date:  1995-01       Impact factor: 3.466

9.  Protein synthesis in Brucella abortus induced during macrophage infection.

Authors:  J Lin; T A Ficht
Journal:  Infect Immun       Date:  1995-04       Impact factor: 3.441

10.  Characterization and genetic complementation of a Brucella abortus high-temperature-requirement A (htrA) deletion mutant.

Authors:  P H Elzer; R W Phillips; M E Kovach; K M Peterson; R M Roop
Journal:  Infect Immun       Date:  1994-10       Impact factor: 3.441
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  23 in total

1.  Identification of a new virulence factor, BvfA, in Brucella suis.

Authors:  Jean-Philippe Lavigne; Gilles Patey; Felix J Sangari; Gisèle Bourg; Michel Ramuz; David O'Callaghan; Sylvie Michaux-Charachon
Journal:  Infect Immun       Date:  2005-09       Impact factor: 3.441

2.  Differential use of the two high-oxygen-affinity terminal oxidases of Brucella suis for in vitro and intramacrophagic multiplication.

Authors:  Séverine Loisel-Meyer; Maria Pilar Jiménez de Bagüés; Stephan Köhler; Jean-Pierre Liautard; Véronique Jubier-Maurin
Journal:  Infect Immun       Date:  2005-11       Impact factor: 3.441

3.  Brucella abortus Depends on l-Serine Biosynthesis for Intracellular Proliferation.

Authors:  Virginia Révora; María Inés Marchesini; Diego J Comerci
Journal:  Infect Immun       Date:  2020-01-22       Impact factor: 3.441

4.  The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell.

Authors:  Stephan Kohler; Vincent Foulongne; Safia Ouahrani-Bettache; Gisele Bourg; Jacques Teyssier; Michel Ramuz; Jean-Pierre Liautard
Journal:  Proc Natl Acad Sci U S A       Date:  2002-11-18       Impact factor: 11.205

5.  Identification of the nik gene cluster of Brucella suis: regulation and contribution to urease activity.

Authors:  V Jubier-Maurin; A Rodrigue; S Ouahrani-Bettache; M Layssac; M A Mandrand-Berthelot; S Köhler; J P Liautard
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

6.  Brucella abortus depends on pyruvate phosphate dikinase and malic enzyme but not on Fbp and GlpX fructose-1,6-bisphosphatases for full virulence in laboratory models.

Authors:  Amaia Zúñiga-Ripa; Thibault Barbier; Raquel Conde-Álvarez; Estrella Martínez-Gómez; Leyre Palacios-Chaves; Yolanda Gil-Ramírez; María Jesús Grilló; Jean-Jacques Letesson; Maite Iriarte; Ignacio Moriyón
Journal:  J Bacteriol       Date:  2014-06-16       Impact factor: 3.490

7.  Global analysis of quorum sensing targets in the intracellular pathogen Brucella melitensis 16 M.

Authors:  Sophie Uzureau; Julien Lemaire; Edouard Delaive; Marc Dieu; Anthoula Gaigneaux; Martine Raes; Xavier De Bolle; Jean-Jacques Letesson
Journal:  J Proteome Res       Date:  2010-06-04       Impact factor: 4.466

8.  Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in mice.

Authors:  P Lestrate; A Dricot; R-M Delrue; C Lambert; V Martinelli; X De Bolle; J-J Letesson; A Tibor
Journal:  Infect Immun       Date:  2003-12       Impact factor: 3.441

9.  Interplay between two RND systems mediating antimicrobial resistance in Brucella suis.

Authors:  Fernando A Martin; Diana M Posadas; Mariela C Carrica; Silvio L Cravero; David O'Callaghan; Angeles Zorreguieta
Journal:  J Bacteriol       Date:  2009-02-06       Impact factor: 3.490

10.  Brucella melitensis invades murine erythrocytes during infection.

Authors:  Marie-Alice Vitry; Delphine Hanot Mambres; Michaël Deghelt; Katrin Hack; Arnaud Machelart; Frédéric Lhomme; Jean-Marie Vanderwinden; Marjorie Vermeersch; Carl De Trez; David Pérez-Morga; Jean-Jacques Letesson; Eric Muraille
Journal:  Infect Immun       Date:  2014-07-07       Impact factor: 3.441
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