Literature DB >> 10567424

Interdomain interaction of merlin isoforms and its influence on intermolecular binding to NHE-RF.

C Gonzalez-Agosti1, T Wiederhold, M E Herndon, J Gusella, V Ramesh.   

Abstract

Merlin, the neurofibromatosis 2 tumor suppressor protein, has two major isoforms with alternate C termini and is related to the ERM (ezrin, radixin, moesin) proteins. Regulation of the ERMs involves intramolecular and/or intermolecular head-to-tail associations between family members. We have determined whether merlin undergoes similar interactions, and our findings indicate that the C terminus of merlin isoform 1 is able to associate with its N-terminal domain in a head-to-tail fashion. However, the C terminus of isoform 2 lacks this property. Similarly, the N terminus of merlin can also associate with C terminus of moesin. We have also explored the effect of merlin self-association on binding to the regulatory cofactor of Na(+)-H(+) exchanger (NHE-RF), an interacting protein for merlin and the ERMs. Merlin isoform 2 captures more NHE-RF than merlin isoform 1 in affinity binding assays, suggesting that in full-length merlin isoform 1, the NHE-RF binding site is masked because of the self-interactions of merlin. Treatment with a phospholipid known to decrease self-association of ERMs enhances the binding of merlin isoform 1 to NHE-RF. Thus, although isoform 1 resembles the ERM proteins, which transition between inactive (closed) and active (open) states, isoform 2 is distinct, existing only in the active (open) state and presumably constitutively more available for interaction with other protein partners.

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Year:  1999        PMID: 10567424     DOI: 10.1074/jbc.274.48.34438

Source DB:  PubMed          Journal:  J Biol Chem        ISSN: 0021-9258            Impact factor:   5.157


  19 in total

1.  Comparative study of ezrin phosphorylation among different tissues: more is good; too much is bad.

Authors:  Lixin Zhu; Jason Hatakeyama; Cheng Chen; Aditi Shastri; Kevin Poon; John G Forte
Journal:  Am J Physiol Cell Physiol       Date:  2008-05-14       Impact factor: 4.249

2.  Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling.

Authors:  Yan Yan; Natalie Denef; Charm Tang; Trudi Schüpbach
Journal:  Development       Date:  2011-03-23       Impact factor: 6.868

3.  Fluorescence resonance energy transfer analysis of merlin conformational changes.

Authors:  Robert F Hennigan; Lauren A Foster; Mary F Chaiken; Timmy Mani; Michelle M Gomes; Andrew B Herr; Wallace Ip
Journal:  Mol Cell Biol       Date:  2010-01       Impact factor: 4.272

4.  Neurofibromatosis 2 (NF2) tumor suppressor merlin inhibits phosphatidylinositol 3-kinase through binding to PIKE-L.

Authors:  Rong Rong; Xiaoling Tang; David H Gutmann; Keqiang Ye
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-14       Impact factor: 11.205

Review 5.  Role of the PDZ-scaffold protein NHERF1/EBP50 in cancer biology: from signaling regulation to clinical relevance.

Authors:  J Vaquero; T H Nguyen Ho-Bouldoires; A Clapéron; L Fouassier
Journal:  Oncogene       Date:  2017-01-09       Impact factor: 9.867

6.  Identification and characterization of putative tumor suppressor NGB, a GTP-binding protein that interacts with the neurofibromatosis 2 protein.

Authors:  Hansoo Lee; Donghwa Kim; Han C Dan; Eric L Wu; Tatiana M Gritsko; Chuanhai Cao; Santo V Nicosia; Erica A Golemis; Wanguo Liu; Domenico Coppola; Steven S Brem; Joseph R Testa; Jin Q Cheng
Journal:  Mol Cell Biol       Date:  2007-01-08       Impact factor: 4.272

7.  The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.

Authors:  H Morrison; L S Sherman; J Legg; F Banine; C Isacke; C A Haipek; D H Gutmann; H Ponta; P Herrlich
Journal:  Genes Dev       Date:  2001-04-15       Impact factor: 11.361

8.  The neurofibromatosis 2 protein product merlin selectively binds F-actin but not G-actin, and stabilizes the filaments through a lateral association.

Authors:  M F James; N Manchanda; C Gonzalez-Agosti; J H Hartwig; V Ramesh
Journal:  Biochem J       Date:  2001-06-01       Impact factor: 3.857

9.  NHE-RF, a merlin-interacting protein, is primarily expressed in luminal epithelia, proliferative endometrium, and estrogen receptor-positive breast carcinomas.

Authors:  A O Stemmer-Rachamimov; T Wiederhold; G P Nielsen; M James; D Pinney-Michalowski; J E Roy; W A Cohen; V Ramesh; D N Louis
Journal:  Am J Pathol       Date:  2001-01       Impact factor: 4.307

Review 10.  Molecular insights into NF2/Merlin tumor suppressor function.

Authors:  Jonathan Cooper; Filippo G Giancotti
Journal:  FEBS Lett       Date:  2014-04-12       Impact factor: 4.124

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