Literature DB >> 10565946

Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses.

K Williams1, S Blake, A Sweeney, J T Singer, B L Nicholson.   

Abstract

A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.

Entities:  

Mesh：

Year:  1999        PMID： 10565946      PMCID： PMC85902     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Expression in Escherichia coli of the large genomic segment of infectious pancreatic necrosis virus.

Authors:  D S Manning; J C Leong
Journal:  Virology       Date:  1990-11       Impact factor: 3.616

2.  Use of a group-reactive and other monoclonal antibodies in an enzyme immunodot assay for identification and presumptive serotyping of aquatic birnaviruses.

Authors:  P Caswell-Reno; V Lipipun; P W Reno; B L Nicholson
Journal:  J Clin Microbiol       Date:  1989-09       Impact factor: 5.948

3.  Detection and identification of aquatic birnaviruses by PCR assay.

Authors:  S L Blake; W B Schill; P E McAllister; M K Lee; J T Singer; B L Nicholson
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948

4.  Differential diagnosis of fish pathogenic rhabdoviruses by reverse transcriptase-dependent polymerase chain reaction.

Authors:  B Bruchhof; O Marquardt; P J Enzmann
Journal:  J Virol Methods       Date:  1995-09       Impact factor: 2.014

5.  Fish cell lines: establishment and characterization of nine cell lines from salmonids.

Authors:  C N Lannan; J R Winton; J L Fryer
Journal:  In Vitro       Date:  1984-09

6.  Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes.

Authors:  P Dobos; B J Hill; R Hallett; D T Kells; H Becht; D Teninges
Journal:  J Virol       Date:  1979-11       Impact factor: 5.103

7.  Physico-chemical and serological characterization of five rhabdoviruses infecting fish.

Authors:  B J Hill
Journal:  J Gen Virol       Date:  1975-06       Impact factor: 3.891

8.  Use of the polymerase chain reaction (PCR) to differentiate serologically similar viral haemorrhagic septicaemia (VHS) virus isolates from Europe and America.

Authors:  K Einer-Jensen; N J Olesen; N Lorenzen; P E Jørgensen
Journal:  Vet Res       Date:  1995       Impact factor: 3.683

9.  Study of a viral-dual infection in rainbow trout (Oncorhynchus mykiss) by seroneutralization, western blot and polymerase chain reaction assays.

Authors:  S Rodríguez; M P Vilas; M Alonso; S I Pérez
Journal:  Microbiologia       Date:  1995-12

10.  The complete genome structure and phylogenetic relationship of infectious hematopoietic necrosis virus.

Authors:  S P Morzunov; J R Winton; S T Nichol
Journal:  Virus Res       Date:  1995-10       Impact factor: 3.303
View more
  9 in total

1.  Characterization of an infectious pancreatic necrosis virus from rainbow trout fry (Onhorhynchus mykiss) in West Ukraine.

Authors:  Yuriy P Rud; Maria I Maistrenko; Leonid P Buchatskiy
Journal:  Virol Sin       Date:  2015-06       Impact factor: 4.327

2.  Establishment and Application of a Multiplex PCR Assay for the Rapid Detection of Rhizoctonia solani Anastomosis Group (AG)-3PT, the Pathogen Causing Potato Black Scurf and Stem Canker.

Authors:  Linda Iradukunda; Yan-Ping Wang; Oswald Nkurikiyimfura; Tian Wang; Li-Na Yang; Jiasui Zhan
Journal:  Pathogens       Date:  2022-05-29

3.  Simultaneous detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, three major fish pathogens, by multiplex PCR.

Authors:  A Del Cerro; I Marquez; J A Guijarro
Journal:  Appl Environ Microbiol       Date:  2002-10       Impact factor: 4.792

4.  Concentration of marine birnavirus from seawater with a glass fiber filter precoated with bovine serum albumin.

Authors:  Shin-Ichiro Kamata; Satoru Suzuki
Journal:  Mar Biotechnol (NY)       Date:  2003 Mar-Apr       Impact factor: 3.619

5.  "Mid-G" region sequences of the glycoprotein gene of Austrian infectious hematopoietic necrosis virus isolates form two lineages within European isolates and are distinct from American and Asian lineages.

Authors:  Jolanta Kolodziejek; Oskar Schachner; Ralf Dürrwald; Muna Latif; Norbert Nowotny
Journal:  J Clin Microbiol       Date:  2007-10-31       Impact factor: 5.948

6.  Detection and characterization of a novel marine birnavirus isolated from Asian seabass in Singapore.

Authors:  Jing Chen; Xinyu Toh; Jasmine Ong; Yahui Wang; Xuan-Hui Teo; Bernett Lee; Pui-San Wong; Denyse Khor; Shin-Min Chong; Diana Chee; Alvin Wee; Yifan Wang; Mee-Keun Ng; Boon-Huan Tan; Taoqi Huangfu
Journal:  Virol J       Date:  2019-05-28       Impact factor: 4.099

7.  Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS).

Authors:  H Soliman; M El-Matbouli
Journal:  Vet Microbiol       Date:  2005-12-27       Impact factor: 3.293

8.  Antiviral Actions of 25-Hydroxycholesterol in Fish Vary With the Virus-Host Combination.

Authors:  Mikolaj Adamek; Jonathan Davies; Alexander Beck; Lisa Jordan; Anna M Becker; Miriam Mojzesz; Krzysztof Rakus; Typhaine Rumiac; Bertrand Collet; Graham Brogden; Keith Way; Sven M Bergmann; Jun Zou; Dieter Steinhagen
Journal:  Front Immunol       Date:  2021-02-24       Impact factor: 7.561

Review 9.  Current and Prospective Methods for Plant Disease Detection.

Authors:  Yi Fang; Ramaraja P Ramasamy
Journal:  Biosensors (Basel)       Date:  2015-08-06
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.