Effects of photoperiod and reproductive responsiveness on pituitary sensitivity to GnRH in male prairie voles (Microtus ochrogaster).

In order to promote survival and reproductive success, many nontropical rodents inhibit reproduction well in advance of winter in response to decreasing day lengths. Male prairie voles (Microtus ochrogaster), small temperate zone rodents, vary in their reproductive response to photoperiod. Some male voles undergo complete gonadal regression when housed in short days (responders) whereas others fail to inhibit reproduction when exposed to short (i. e., <12 h light/day) day lengths (nonresponders). Previous research has shown that phenotypic variation in reproductive response is reflected at the level of the hypothalamic gonadotropin-releasing hormone (GnRH) neuronal system. The present study sought to determine if photoperiod or reproductive condition alters pituitary responsiveness to a GnRH challenge. Animals were housed in either long (LD 16:8) or short (LD 8:16) photoperiods for 10 weeks. Subsequently, short-day voles were separated into responders and nonresponders based on testicular size. To reduce the influence of endogenous testosterone on luteinizing hormone concentrations, half of the animals in each group were castrated. All animals were injected (i.p.) with either 100 or 50 ng of GnRH and a blood sample was collected after 15 min. Although castration resulted in a significant increase in LH concentrations (P < 0.05), neither photoperiod nor reproductive condition affected LH concentrations in response to a GnRH challenge (P > 0.05). Taken together, these data support the hypothesis that short photoperiods lead to reproductive inhibition by acting at the level of the hypothalamus rather than the pituitary. Copyright 1999 Academic Press.