Literature DB >> 10556711

New PCR method to differentiate species in the Aspergillus niger aggregate.

F Accensi1, J Cano, L Figuera, M L Abarca, F J Cabañes.   

Abstract

The DNA that encodes the 5.8S gene of the ribosomal RNA and the two intergenic spacers ITS1 and ITS2 of the two proposed type strains of the Aspergillus niger aggregate (A. niger and Aspergillus tubingensis) have been sequenced. By comparison of sequences we have found that both species could be differentiated by RsaI digestion of the PCR products of the mentioned regions. This method could be a useful tool in the identification of strains of the A. niger aggregate, especially in studies that involve a large number of isolates.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 10556711     DOI： 10.1111/j.1574-6968.1999.tb08795.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  11 in total

1.  High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray.

Authors:  Aihua Huang; Jun-Wen Li; Zhi-Qiang Shen; Xin-Wei Wang; Min Jin
Journal:  J Clin Microbiol       Date:  2006-09       Impact factor: 5.948

2.  Study of Spanish grape mycobiota and ochratoxin A production by Isolates of Aspergillus tubingensis and other members of Aspergillus section Nigri.

Authors:  Angel Medina; Rufino Mateo; Laura López-Ocaña; Francisco Manuel Valle-Algarra; Misericordia Jiménez
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

3.  Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections.

Authors:  C Ferrer; F Colom; S Frasés; E Mulet; J L Abad; J L Alió
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

4.  New morphological criteria and molecular characterization of black aspergilli aggregate from corn, sorghum and wheat grains.

Authors:  Youssuf A Gherbawy; Thanaa A Maghraby; Lamiaa H A Hamza; Eman G A M El-Dawy
Journal:  Arch Microbiol       Date:  2020-09-21       Impact factor: 2.552

5.  Culture-positive invasive aspergillosis in a medical center in Taiwan, 2000-2009.

Authors:  H-C Hsiue; T-H Wu; T-C Chang; Y-C Hsiue; Y-T Huang; P-I Lee; P-R Hsueh
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-10-14       Impact factor: 3.267

6.  Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species.

Authors:  Hans P Hinrikson; Steven F Hurst; Timothy J Lott; David W Warnock; Christine J Morrison
Journal:  J Clin Microbiol       Date:  2005-05       Impact factor: 5.948

7.  Utility of microsatellite markers and amplified fragment length polymorphism in the study of potentially ochratoxigenic black aspergilli.

Authors:  Alexandre Esteban; Su-lin L Leong; Ailsa D Hocking; M Lourdes Abarca; F Javier Cabañes; Nai Tran-Dinh
Journal:  Curr Microbiol       Date:  2008-07-31       Impact factor: 2.188

Review 8.  The black Aspergillus species of maize and peanuts and their potential for mycotoxin production.

Authors:  Edwin R Palencia; Dorothy M Hinton; Charles W Bacon
Journal:  Toxins (Basel)       Date:  2010-03-24       Impact factor: 4.546

Review 9.  Ochratoxin a: general overview and actual molecular status.

Authors:  André el Khoury; Ali Atoui
Journal:  Toxins (Basel)       Date:  2010-03-29       Impact factor: 4.546

10.  Ochratoxigenic black species of Aspergilli in grape fruits of northern Italy identified by an improved PCR-RFLP procedure.

Authors:  Davide Spadaro; Subban Patharajan; Alessia Lorè; Angelo Garibaldi; Maria Lodovica Gullino
Journal:  Toxins (Basel)       Date:  2012-01-30       Impact factor: 4.546
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.