| Literature DB >> 10556701 |
Abstract
A simple, efficient, sensitive, reproducible and high throughput assay for measuring the cytoadhesion of Plasmodium falciparum-infected red blood cells (human malaria) is described. The assay format uses 96-well microplates, with the number of P. falciparum parasitized erythrocytes bound determined by measuring Plasmodium specific lactic dehydrogenase activity colorimetrically (absorbance at 655 nm) using the 3-acetylpyridine analog of nicotinamide adenine dinucleotide, nitro blue tetrazolium and diaphorase. The results of the described microplate assay were found to be comparable to those using microscopic analysis but much less time consuming.Entities:
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Year: 1999 PMID： 10556701 DOI： 10.1016/s0022-1759(99)00098-8
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303