Supercritical fluid extraction of microcystins from cyanobacteria.

A new method for the fast extraction of microcystins RR and LR in cyanobacterium was developed using supercritical CO2. The microcystins were successfully extracted with aqueous methanol modified supercritical fluid (90% CO2, 9.0% methanol, 1.0% water). The method developed here has several advantages over solid-phase extraction sample preparation for the analysis of microcystins. Sample handling steps are minimized, thus reducing possible losses of analytes and saving analysis time. No organic solvent extractions are involved in this method, and no cleanup steps are employed.