Literature DB >> 10546536

Liquid chromatography-tandem mass spectrometry of some anabolic steroids.

P E Joos1, M Van Ryckeghem.   

Abstract

In this paper a procedure is described for the analysis of 36 anabolic steroids, regularly found in kidney fat matrixes. After preparative HPLC is carried out, six fractions, containing the different steroids, were obtained. These fractions were then separated and on-line mass-analyzed, using APcI LC/MS. When used in tandem mass spectrometry (MRM) mode, it is possible to obtain detection limits below 1 ppb for all steroids and below 0.1 ppb for most of them. As such, it is possible to meet the regulations for detection of these anabolic steroids. To verify the influence of matrixes, real-world samples were analyzed in the same manner. This resulted in almost analog detection limits.

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Year:  1999        PMID： 10546536     DOI： 10.1021/ac981073s

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  7 in total

1.  An improved micro-method for the measurement of steroid profiles by APPI-LC-MS/MS and its use in assessing diurnal effects on steroid concentrations and optimizing the diagnosis and treatment of adrenal insufficiency and CAH.

Authors:  Brian R Stolze; Verena Gounden; Jianghong Gu; Elizabeth A Elliott; Likhona S Masika; Brent S Abel; Deborah P Merke; Monica C Skarulis; Steven J Soldin
Journal:  J Steroid Biochem Mol Biol       Date:  2015-12-22       Impact factor: 4.292

2.  Steroid hormone levels in pregnancy and 1 year postpartum using isotope dilution tandem mass spectrometry.

Authors:  Offie P Soldin; Tiedong Guo; Elisabete Weiderpass; Rochelle E Tractenberg; Leena Hilakivi-Clarke; Steven J Soldin
Journal:  Fertil Steril       Date:  2005-09       Impact factor: 7.329

Review 3.  Atmospheric pressure chemical ionization mass spectrometry for analysis of lipids.

Authors:  W C Byrdwell
Journal:  Lipids       Date:  2001-04       Impact factor: 1.880

4.  The bottom-up solution to the triacylglycerol lipidome using atmospheric pressure chemical ionization mass spectrometry.

Authors:  William Craig Byrdwell
Journal:  Lipids       Date:  2005-04       Impact factor: 1.880

5.  Quantitation of lanosterol and its major metabolite FF-MAS in an inhibition assay of CYP51 by azoles with atmospheric pressure photoionization based LC-MS/MS.

Authors:  Eva R Trösken; Ellen Straube; Werner K Lutz; Wolfgang Völkel; Christopher Patten
Journal:  J Am Soc Mass Spectrom       Date:  2004-08       Impact factor: 3.109

Review 6.  Steroid hormones: relevance and measurement in the clinical laboratory.

Authors:  Jennifer P Holst; Offie P Soldin; Tiedong Guo; Steven J Soldin
Journal:  Clin Lab Med       Date:  2004-03       Impact factor: 1.935

7.  Detection and analysis of 17 steroid hormones by ultra-high-performance liquid chromatography-electrospray ionization mass spectrometry (UHPLC-MS) in different sex and maturity stages of Antarctic krill (Euphausia superba Dana).

Authors:  Xiangning Han; Daicheng Liu
Journal:  PLoS One       Date:  2019-03-11       Impact factor: 3.240
  7 in total

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