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Literature DB >> 10543768

Overexpression of Erg11p by the regulatable GAL1 promoter confers fluconazole resistance in Saccharomyces cerevisiae.

D P Kontoyiannis¹, N Sagar, K D Hirschi.

Abstract

The contribution of the dosage of target enzyme P-450 14alpha-demethylase (14alphaDM) to fluconazole resistance in both Candida albicans and Saccharomyces cerevisiae remains unclear. Here, we show that overexpression of Saccharomyces P-450 14alphaDM in S. cerevisiae, under the control of the regulatable promoter GAL1, results in azole resistance.

Entities: Chemical Gene Species

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Year: 1999 PMID： 10543768 PMCID： PMC89564

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

9 in total

1. The mutation T315A in Candida albicans sterol 14alpha-demethylase causes reduced enzyme activity and fluconazole resistance through reduced affinity.

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Review 2. Clinical, cellular, and molecular factors that contribute to antifungal drug resistance.

Authors: T C White; K A Marr; R A Bowden
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Authors: D R Kirsch; M H Lai; J O'Sullivan
Journal: Gene Date: 1988-09-07 Impact factor: 3.688

Review 4. Molecular mechanisms of azole resistance in fungi.

Authors: T Joseph-Horne; D W Hollomon
Journal: FEMS Microbiol Lett Date: 1997-04-15 Impact factor: 2.742

5. Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus.

Authors: T C White
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Review 6. Molecular genetic analysis of azole antifungal mode of action.

Authors: S L Kelly; A Arnoldi; D E Kelly
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7. Amino acid substitutions in the cytochrome P-450 lanosterol 14alpha-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents.

Authors: D Sanglard; F Ischer; L Koymans; J Bille
Journal: Antimicrob Agents Chemother Date: 1998-02 Impact factor: 5.191

8. Distinct patterns of gene expression associated with development of fluconazole resistance in serial candida albicans isolates from human immunodeficiency virus-infected patients with oropharyngeal candidiasis.

Authors: J L Lopez-Ribot; R K McAtee; L N Lee; W R Kirkpatrick; T C White; D Sanglard; T F Patterson
Journal: Antimicrob Agents Chemother Date: 1998-11 Impact factor: 5.191

9. Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in yeast.

Authors: H Liu; J Krizek; A Bretscher
Journal: Genetics Date: 1992-11 Impact factor: 4.562

9 in total

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4. An integrated approach for identification and target validation of antifungal compounds active against Erg11p.

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5. A CTG Clade Candida Yeast Genetically Engineered for the Genotype-Phenotype Characterization of Azole Antifungal Resistance in Human-Pathogenic Yeasts.

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