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Literature DB >> 10535943

Ku is associated with the telomere in mammals.

H L Hsu¹, D Gilley, E H Blackburn, D J Chen.

Abstract

Telomeres are specialized DNA/protein complexes that comprise the ends of eukaryotic chromosomes. The highly expressed Ku heterodimer, composed of 70 and 80 K(d) subunits (Ku70 and Ku80), is the high-affinity DNA binding component of the DNA-dependent protein kinase. Ku is critical for nonhomologous DNA double-stranded break repair and site-specific recombination of V(D)J gene segments. Ku also plays an important role in telomere maintenance in yeast. Herein, we report, using an in vivo crosslinking method, that human and hamster telomeric DNAs specifically coimmunoprecipitate with human Ku80 after crosslinking. Localization of Ku to the telomere does not depend on the DNA-dependent protein kinase catalytic component. These findings suggest a direct link between Ku and the telomere in mammalian cells.

Entities: Gene Species

Mesh：

Substances：

Year: 1999 PMID： 10535943 PMCID： PMC22947 DOI： 10.1073/pnas.96.22.12454

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

41 in total

Review 1. Telomeres--unsticky ends.

Authors: D Shore
Journal: Science Date: 1998-09-18 Impact factor: 47.728

Review 2. Effects of Alu insertions on gene function.

Authors: M N Szmulewicz; G E Novick; R J Herrera
Journal: Electrophoresis Date: 1998-06 Impact factor: 3.535

3. Length control of human telomeres.

Authors: T de Lange
Journal: Cancer J Sci Am Date: 1998-05

4. Telomeres and double-strand breaks: trying to make ends meet.

Authors: A Bertuch; V Lundblad
Journal: Trends Cell Biol Date: 1998-09 Impact factor: 20.808

5. Evidence of a differential organization of chromatin containing terminal or interstitial (TTAGGG)n repeats by in situ digestion with nucleases.

Authors: J L Fernández; V J Goyanes; J Ramiro-Díaz; J Gosálvez
Journal: Cytogenet Cell Genet Date: 1998

6. Yeast Ku as a regulator of chromosomal DNA end structure.

Authors: S Gravel; M Larrivée; P Labrecque; R J Wellinger
Journal: Science Date: 1998-05-01 Impact factor: 47.728

7. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening.

Authors: J Zhu; H Wang; J M Bishop; E H Blackburn
Journal: Proc Natl Acad Sci U S A Date: 1999-03-30 Impact factor: 11.205

8. Elongated telomeres in scid mice.

Authors: P Hande; P Slijepcevic; A Silver; S Bouffler; P van Buul; P Bryant; P Lansdorp
Journal: Genomics Date: 1999-03-01 Impact factor: 5.736

9. Tankyrase, a poly(ADP-ribose) polymerase at human telomeres.

Authors: S Smith; I Giriat; A Schmitt; T de Lange
Journal: Science Date: 1998-11-20 Impact factor: 47.728

10. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization.

Authors: C M Counter; W C Hahn; W Wei; S D Caddle; R L Beijersbergen; P M Lansdorp; J M Sedivy; R A Weinberg
Journal: Proc Natl Acad Sci U S A Date: 1998-12-08 Impact factor: 11.205

92 in total

1. Involvement of cellular double-stranded DNA break binding proteins in processing of the recombinant adeno-associated virus genome.

Authors: L Zentilin; A Marcello; M Giacca
Journal: J Virol Date: 2001-12 Impact factor: 5.103

2. Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction.

Authors: Juren Huang; William S Dynan
Journal: Nucleic Acids Res Date: 2002-02-01 Impact factor: 16.971

3. Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2.

Authors: Katia Ancelin; Michele Brunori; Serge Bauwens; Catherine-Elaine Koering; Christine Brun; Michelle Ricoul; Jean-Patrick Pommier; Laure Sabatier; Eric Gilson
Journal: Mol Cell Biol Date: 2002-05 Impact factor: 4.272

Ku is associated with the telomere in mammals.

Review 1. Telomeres--unsticky ends.

Review 2. Effects of Alu insertions on gene function.

3. Length control of human telomeres.

4. Telomeres and double-strand breaks: trying to make ends meet.

5. Evidence of a differential organization of chromatin containing terminal or interstitial (TTAGGG)n repeats by in situ digestion with nucleases.

6. Yeast Ku as a regulator of chromosomal DNA end structure.

7. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening.

8. Elongated telomeres in scid mice.

9. Tankyrase, a poly(ADP-ribose) polymerase at human telomeres.

10. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization.

1. Involvement of cellular double-stranded DNA break binding proteins in processing of the recombinant adeno-associated virus genome.

2. Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction.

3. Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2.

4. T-loop assembly in vitro involves binding of TRF2 near the 3' telomeric overhang.

5. DNA end-binding specificity of human Rad50/Mre11 is influenced by ATP.

6. Rap1-independent telomere attachment and bouquet formation in mammalian meiosis.

7. Protection of telomeres by the Ku protein in fission yeast.

8. Ku acts in a unique way at the mammalian telomere to prevent end joining.

9. Telomere instability in a human tumor cell line expressing a dominant-negative WRN protein.

10. Diverse patterns of the tandem repeats organization in rye chromosomes.