Literature DB >> 10527726

Characterization of heterologous protein-protein interactions using analytical ultracentrifugation.

G Rivas1, W Stafford, A P Minton.   

Abstract

Methods for quantitative characterization of heterologous protein-protein interactions by means of analytical ultracentrifugation (AUC) include sedimentation equilibrium, tracer sedimentation equilibrium, sedimentation velocity, and analytical band sedimentation. Fundamental principles governing the behavior of macromolecules in a centrifugal field are summarized, and the application of these principles to the interpretation of data obtained from each type of experiment is reviewed. Instrumentation and software for the acquisition and analysis of data obtained from different types of AUC experiments are described. Copyright 1999 Academic Press.

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Year:  1999        PMID： 10527726     DOI： 10.1006/meth.1999.0851

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  22 in total

1.  Analysis of PKR activation using analytical ultracentrifugation.

Authors:  James L Cole
Journal:  Macromol Biosci       Date:  2010-07-07       Impact factor: 4.979

Review 2.  Modern analytical ultracentrifugation in protein science: a tutorial review.

Authors:  Jacob Lebowitz; Marc S Lewis; Peter Schuck
Journal:  Protein Sci       Date:  2002-09       Impact factor: 6.725

Review 3.  Sedimentation equilibrium: a valuable tool to study homologous and heterogeneous interactions of proteins or proteins and nucleic acids.

Authors:  Joachim Behlke; Otto Ristau
Journal:  Eur Biophys J       Date:  2003-05-29       Impact factor: 1.733

4.  Analysis of heterogeneous interactions.

Authors:  James L Cole
Journal:  Methods Enzymol       Date:  2004       Impact factor: 1.600

5.  Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB.

Authors:  Gonzalo Durante-Rodríguez; J Andrés Valderrama; José Miguel Mancheño; Germán Rivas; Carlos Alfonso; Ernesto Arias-Palomo; Oscar Llorca; José Luis García; Eduardo Díaz; Manuel Carmona
Journal:  J Biol Chem       Date:  2010-09-08       Impact factor: 5.157

Review 6.  Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge.

Authors:  Lance M Hellman; Chunxia Zhao; Manana Melikishvili; Xiaorong Tao; James E Hopper; Sidney W Whiteheart; Michael G Fried
Journal:  Methods       Date:  2010-12-25       Impact factor: 3.608

7.  Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation.

Authors:  Andrea Balbo; Kenneth H Minor; Carlos A Velikovsky; Roy A Mariuzza; Cynthia B Peterson; Peter Schuck
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-21       Impact factor: 11.205

8.  Sedimentation velocity analysis of heterogeneous protein-protein interactions: Lamm equation modeling and sedimentation coefficient distributions c(s).

Authors:  Julie Dam; Carlos A Velikovsky; Roy A Mariuzza; Claus Urbanke; Peter Schuck
Journal:  Biophys J       Date:  2005-04-29       Impact factor: 4.033

9.  Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation.

Authors:  Patrick H Brown; Peter Schuck
Journal:  Biophys J       Date:  2006-03-24       Impact factor: 4.033

10.  Inducer-modulated cooperative binding of the tetrameric CggR repressor to operator DNA.

Authors:  Silvia Zorrilla; Thierry Doan; Carlos Alfonso; Emmanuel Margeat; Alvaro Ortega; Germán Rivas; Stéphane Aymerich; Catherine A Royer; Nathalie Declerck
Journal:  Biophys J       Date:  2007-02-09       Impact factor: 4.033
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