Literature DB >> 1052544

Metrizamide density gradients of sea urchin chromatin: fractions rich and poor in nascent DNA.

A Levy, K M Jakob, B Moav.   

Abstract

A crude, lightly sheared chromatin preparation obtained from a mixture of [methyl-3H] thymidine pulse and [2-14C] thymidine long-labeled sea urchin embryos (swimming blastulae), was centrifuged in metrizamide to form an isopycnic gradient. The buoyant density of the 3H pulse labeled chromatin was slightly higher than that of the 14C labeled bulk chromatin. The 3H/14C ratios in the higher and lower density regions of the overlapping radioactivity peaks, indicated the presence of fractions rich and poor in nascent DNA in these two density regions. After 15 min chase, the difference disappeared, indicating that the chromatin fractions with nascent DNA have a half-life shorter than 15 min.

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Year:  1975        PMID： 1052544      PMCID： PMC343597          DOI： 10.1093/nar/2.12.2299

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  21 in total

1.  Covalent linkage between RNA and nascent DNA in the slime mold, Physarum polycephalum.

Authors:  M A Waqar; J A Huberman
Journal:  Biochim Biophys Acta       Date:  1975-04-02

2.  SH compounds in mitosis. II. The effect of mercaptoethanol on the structure of the mitotic apparatus in sea urchin eggs.

Authors:  D MAZIA; A M ZIMMERMAN
Journal:  Exp Cell Res       Date:  1958-08       Impact factor: 3.905

3.  Buoyant densities and hydration of nucleic acids, proteins and nucleoprotein complexes in metrizamide.

Authors:  G D Birnie; D Rickwood; A Hell
Journal:  Biochim Biophys Acta       Date:  1973-12-07

4.  Transcription of chromatin in vitro.

Authors:  H Cedar; G Felsenfeld
Journal:  J Mol Biol       Date:  1973-06-25       Impact factor: 5.469

Review 5.  Units of DNA replication in chromosomes of eukaryotes.

Authors:  J H Taylor
Journal:  Int Rev Cytol       Date:  1974

6.  Bi-directional initiation of DNA synthesis in developing chick erythroblasts.

Authors:  H Weintraub
Journal:  Nat New Biol       Date:  1972-04-19

7.  Partial purification of the template-active fraction of chromatin: a preliminary report.

Authors:  J M Gottesfeld; W T Garrard; G Bagi; R F Wilson; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

8.  RNA in nascent DNA from cultured human lymphocytes.

Authors:  R M Fox; J Mendelsohn; E Barbosa; M Goulian
Journal:  Nat New Biol       Date:  1973-10-24

9.  Interphase chromosomal deoxyribonucleoprotein isolated as a discrete structure from cultured cells.

Authors:  R Hancock
Journal:  J Mol Biol       Date:  1974-07-05       Impact factor: 5.469

10.  The isolation of nuclei from eggs and embryos of the sea urchin.

Authors:  R T HINEGARDNER
Journal:  J Cell Biol       Date:  1962-12       Impact factor: 10.539
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  4 in total

Review 1.  Chromatin replication revealed by studies of animal cells and papovaviruses (simian virus 40 and polyoma virus).

Authors:  C Crémisi
Journal:  Microbiol Rev       Date:  1979-09

2.  Isolation of newly replicated chromatin by using shallow metrizamide gradients.

Authors:  R F Murphy; R B Wallace; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

3.  Metrizamide dissociates nuclear particles containing heterogeneous nuclear RNA.

Authors:  R Gattoni; J Stevenin; M Jacob
Journal:  Nucleic Acids Res       Date:  1977-11       Impact factor: 16.971

4.  Changes in chromatin structure at the replication fork. II The DNPs containing nascent DNA and a transient chromatin modification detected by DNAase I.

Authors:  G Galili; A Levy; K M Jakob
Journal:  Nucleic Acids Res       Date:  1981-08-25       Impact factor: 16.971
  4 in total

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