Literature DB >> 1052528

A new method of DNA denaturation mapping.

D Johnson.   

Abstract

Described is a new method for DNA denaturation mapping utilizing glyoxal (ethanedial) to stabilize the denatured regions. The extent of glyoxal reaction can be easily and sensitively measured using an assay based on the intercalation of ethidium into duplex DNA. Thus denturation maps can be produced in a controlled way under a wide variety of conditions.

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Year:  1975        PMID： 1052528      PMCID： PMC343571          DOI： 10.1093/nar/2.11.2049

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  A new method for mapping nucleic acid sequence homology by electron microscopy.

Authors:  M D Bick; C A Thomas
Journal:  Nucleic Acids Res       Date:  1975-04       Impact factor: 16.971

2.  An electron microscope study of Sindbis virus RNA.

Authors:  M T Hsu; H J Kung; N Davidson
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

3.  Native and denatured DNA, cross-linked and palindromic DNA and circular covalently-closed DNA analysed by a sensitive fluorometric procedure.

Authors:  A R Morgan; D E Pulleyblank
Journal:  Biochem Biophys Res Commun       Date:  1974-11-27       Impact factor: 3.575

4.  A fluorescence assay for DNA with covalently linked complementary sequences.

Authors:  A R Morgan; V Paetkau
Journal:  Can J Biochem       Date:  1972-02

5.  The reaction of glyoxal with nucleic acid components. 3. Kinetics of the reaction with monomers.

Authors:  N E Broude; E I Budowsky
Journal:  Biochim Biophys Acta       Date:  1971-12-30

6.  Denaturation maps of the left and right sides of the lambda DNA molecule determined by electron microscopy.

Authors:  R B Inman
Journal:  J Mol Biol       Date:  1967-08-28       Impact factor: 5.469

7.  A denaturation map of the lambda phage DNA molecule determined by electron microscopy.

Authors:  R B Inman
Journal:  J Mol Biol       Date:  1966-07       Impact factor: 5.469

8.  The reaction of guanine derivatives with 1,2-dicarbonyl compounds.

Authors:  R Shapiro; J Hachmann
Journal:  Biochemistry       Date:  1966-09       Impact factor: 3.162
  8 in total
  6 in total

1.  Electron microscopy of DNA crosslinked with trimethylpsoralen: test of the secondary structure of eukaryotic inverted repeat sequences.

Authors:  T R Cech; M L Pardue
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

2.  DNA overstretching in the presence of glyoxal: structural evidence of force-induced DNA melting.

Authors:  Leila Shokri; Micah J McCauley; Ioulia Rouzina; Mark C Williams
Journal:  Biophys J       Date:  2008-04-18       Impact factor: 4.033

3.  Determination of the stereostructure of the product of Tn3 resolvase by a general method.

Authors:  S A Wasserman; N R Cozzarelli
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

4.  Enzymatic properties of the bacteriophage phi X174 A protein on superhelical phi X174 DNA: a model for the termination of the rolling circle DNA replication.

Authors:  A van der Ende; S A Langeveld; R Teertstra; G A van Arkel; P J Weisbeek
Journal:  Nucleic Acids Res       Date:  1981-05-11       Impact factor: 16.971

5.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

6.  Force spectroscopy and fluorescence microscopy of dsDNA-YOYO-1 complexes: implications for the structure of dsDNA in the overstretching region.

Authors:  Chandrashekhar U Murade; Vinod Subramaniam; Cees Otto; Martin L Bennink
Journal:  Nucleic Acids Res       Date:  2010-02-02       Impact factor: 16.971
  6 in total

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