Literature DB >> 10523590

Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei.

S Korbsrisate1, N Suwanasai, A Leelaporn, T Ezaki, Y Kawamura, S Sarasombath.   

Abstract

We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic PLC from Pseudomonas aeruginosa. The expressed PC-PLC was heat stable, nonhemolytic for sheep erythrocytes, and active between pH 2 and 8. Western blot analysis with sera from melioidosis patients indicated that they produced immunoglobulin M antibodies against this PC-PLC protein.

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Year:  1999        PMID： 10523590      PMCID： PMC85747     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa.

Authors:  R M Ostroff; A I Vasil; M L Vasil
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

2.  Production of hemolysin and other extracellular enzymes by clinical isolates of Pseudomonas pseudomallei.

Authors:  L R Ashdown; J M Koehler
Journal:  J Clin Microbiol       Date:  1990-10       Impact factor: 5.948

3.  A comparative study of Pseudomonas pseudomallei and Bacillus mallei.

Authors:  M S Redfearn; N J Palleroni; R Y Stanier
Journal:  J Gen Microbiol       Date:  1966-05

Review 4.  Melioidosis: the tip of the iceberg?

Authors:  D A Dance
Journal:  Clin Microbiol Rev       Date:  1991-01       Impact factor: 26.132

5.  Gene cloning shows the alpha-toxin of Clostridium perfringens to contain both sphingomyelinase and lecithinase activities.

Authors:  B Saint-Joanis; T Garnier; S T Cole
Journal:  Mol Gen Genet       Date:  1989-11

6.  Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand.

Authors:  W Chaowagul; N J White; D A Dance; Y Wattanagoon; P Naigowit; T M Davis; S Looareesuwan; N Pitakwatchara
Journal:  J Infect Dis       Date:  1989-05       Impact factor: 5.226

7.  Characterization of hemolysin in extracellular products of Pseudomonas cepacia.

Authors:  T Nakazawa; Y Yamada; M Ishibashi
Journal:  J Clin Microbiol       Date:  1987-02       Impact factor: 5.948

8.  Mutations in the hemolytic-phospholipase C operon result in decreased virulence of Pseudomonas aeruginosa PAO1 grown under phosphate-limiting conditions.

Authors:  R M Ostroff; B Wretlind; M L Vasil
Journal:  Infect Immun       Date:  1989-05       Impact factor: 3.441

9.  Enzyme-linked immunosorbent assay for the diagnosis of clinical and subclinical melioidosis.

Authors:  L R Ashdown; R W Johnson; J M Koehler; C A Cooney
Journal:  J Infect Dis       Date:  1989-08       Impact factor: 5.226

10.  Survival of Pseudomonas pseudomallei in human phagocytes.

Authors:  S Pruksachartvuthi; N Aswapokee; K Thankerngpol
Journal:  J Med Microbiol       Date:  1990-02       Impact factor: 2.472
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  4 in total

Review 1.  Melioidosis: epidemiology, pathophysiology, and management.

Authors:  Allen C Cheng; Bart J Currie
Journal:  Clin Microbiol Rev       Date:  2005-04       Impact factor: 26.132

2.  Genome-wide expression analysis of Burkholderia pseudomallei infection in a hamster model of acute melioidosis.

Authors:  Apichai Tuanyok; Marina Tom; John Dunbar; Donald E Woods
Journal:  Infect Immun       Date:  2006-10       Impact factor: 3.441

3.  Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system.

Authors:  Magdy E Mahfouz; T Hilton Grayson; David A B Dance; Martyn L Gilpin
Journal:  BMC Microbiol       Date:  2006-08-07       Impact factor: 3.605

4.  Genomic islands from five strains of Burkholderia pseudomallei.

Authors:  Apichai Tuanyok; Benjamin R Leadem; Raymond K Auerbach; Stephen M Beckstrom-Sternberg; James S Beckstrom-Sternberg; Mark Mayo; Vanaporn Wuthiekanun; Thomas S Brettin; William C Nierman; Sharon J Peacock; Bart J Currie; David M Wagner; Paul Keim
Journal:  BMC Genomics       Date:  2008-11-27       Impact factor: 3.969
  4 in total

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