Cloning and analysing of 5' flanking region of Xenopus organizer gene noggin.

Xenopus organizer specific gene Noggin possesses nearly all the characteristic properties of the action of organizer to specify the embryonic body axis. To analyze how the maternal inherited factors control its expression pattern, we cloned the 5' regulatory region of noggin gene. The 1.5 kb upstream sequence could direct reporter gene to express in vivo and data from deletion analysis indicated that a 229 base pair fragment is essential for activating noggin expression. We further demonstrated that the response elements within this regulatory region were indeed under the control of growth factor activin and Wnt signaling pathway components.