| Literature DB >> 10520260 |
O M Hanniffy1, A S Shashkov, A P Moran, M M Prendergast, S N Senchenkova, Y A Knirel, A V Savage.
Abstract
A neutral polysaccharide was obtained by hot phenol-water extraction of biomass from Campylobacter jejuni 176.83 and subsequently separated from acid-liberated core oligosaccharide of lipopolysaccharide by sequential GPC on Bio-Gel P6 and TSK-40 columns. All sugar components of the trisaccharide repeating unit of the polysaccharide were found to be of the furanose ring form. The major trisaccharide contained beta-L-arabinose, 6-deoxy-beta-D-altro-heptose (beta-D-6d-altHep) and 6-deoxy-beta-L-altrose (beta-L-6d-Alt), whereas in the minor trisaccharide the beta-L-6d-Alt is replaced by its C-5 epimer alpha-D-Fuc. On the basis of 1H and 13C NMR spectroscopic studies, including 2D ROESY, HMQC and HMQC-TOCSY experiments, the following structures of the repeating units were established: [formula: see text]Entities:
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Year: 1999 PMID： 10520260 DOI： 10.1016/s0008-6215(99)00129-9
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104