| Literature DB >> 10519106 |
X Cao1, Y Tian, T Zhang, X Li, Y Ito.
Abstract
High-speed counter-current chromatography (HSCCC) was applied to the semipreparative separation and purification of puerarin and related isoflavones from a crude extract of Pueraria lobata. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v). Using the above solvent system the preparative HSCCC was successfully performed yielding six relatively pure isoflavones including puerarin from 80 mg of the crude extract in one-step separation.Entities:
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Year: 1999 PMID： 10519106 DOI： 10.1016/s0021-9673(99)00715-3
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759