| Literature DB >> 10518697 |
Abstract
The association between liposome-DNA complexes (lipoplexes) and targeted cell membranes is a limiting step of cationic liposome-mediated transfection. A novel technique was developed where lipoplex-cell membrane association is enhanced by the addition of 2-6% polyethylene glycol (PEG) to the transfection media. Lipoplex-cell association was found to increase up to 100 times in the presence of PEG. Transfection increased correspondingly in the presence of PEG. This increase was found in several cell lines. These results show that lipoplex adsorption to cell membranes is a critical step in liposome-mediated transfection. This step can be facilitated by PEG-induced particle aggregation.Entities:
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Year: 1999 PMID： 10518697 DOI： 10.1016/s0005-2736(99)00132-7
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002