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Literature DB >> 10517796

Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.

J Zimmerberg¹, J R Coorssen, S S Vogel, P S Blank.

Abstract

This paper reviews recent work in our laboratory on the mechanism of calcium-triggered exocytosis. Upon echinoderm egg fertilization, cortical secretory vesicle exocytosis is massive and synchronous. By combining physiological and molecular analyses with a variety of purified membrane isolates containing secretory vesicles that fuse to the plasma membrane or each other, we have characterized the final steps of this calcium-triggered exocytosis. Our kinetic analysis led to a functional definition of the fusion complex whose activation by calcium follows Poisson statistics. The properties of this complex are compared with the properties of the heterotrimeric SNARE protein complex that is present in the cortical vesicle system. Our data do not support the hypothesis that this particular heterotrimeric complex is by itself the biological fusogen.

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Year: 1999 PMID： 10517796 PMCID： PMC2269559 DOI： 10.1111/j.1469-7793.1999.00015.x

Source DB: PubMed Journal: J Physiol ISSN： 0022-3751 Impact factor: 5.182

46 in total

1. The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.

Authors: V D Vacquier
Journal: Dev Biol Date: 1975-03 Impact factor: 3.582

2. Influence of ATP and calcium on the cortical reaction in sea urchin eggs.

Authors: P F Baker; M J Whitaker
Journal: Nature Date: 1978-11-30 Impact factor: 49.962

Review 3. Calcium control of exocytosis and endocytosis in bovine adrenal medullary cells.

Authors: P F Baker; D E Knight
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1981-12-18 Impact factor: 6.237

4. Fusion complex formation protects synaptobrevin against proteolysis by tetanus toxin light chain.

Authors: L L Pellegrini; V O'Connor; H Betz
Journal: FEBS Lett Date: 1994-10-24 Impact factor: 4.124

5. Cortical vesicle exocytosis in isolated cortices of sea urchin eggs: description of a turbidometric assay and its utilization in studying effects of different media on discharge.

Authors: H Sasaki; D Epel
Journal: Dev Biol Date: 1983-08 Impact factor: 3.582

6. Release of granule contents from sea urchin egg cortices. New assay procedures and inhibition by sulfhydryl-modifying reagents.

Authors: J G Haggerty; R C Jackson
Journal: J Biol Chem Date: 1983-02-10 Impact factor: 5.157

7. A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion.

Authors: T Söllner; M K Bennett; S W Whiteheart; R H Scheller; J E Rothman
Journal: Cell Date: 1993-11-05 Impact factor: 41.582

Review 8. Vesicle fusion from yeast to man.

Authors: S Ferro-Novick; R Jahn
Journal: Nature Date: 1994-07-21 Impact factor: 49.962

9. Calcium-dependent exocytosis in an in vitro secretory granule plasma membrane preparation from sea urchin eggs and the effects of some inhibitors of cytoskeletal function.

Authors: M J Whitaker; P F Baker
Journal: Proc R Soc Lond B Biol Sci Date: 1983-07-22

10. Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. I. Discharge of vesicular contents across the planar membrane.

Authors: J Zimmerberg; F S Cohen; A Finkelstein
Journal: J Gen Physiol Date: 1980-03 Impact factor: 4.086

11 in total

10. Combined targeted Omic and Functional Assays Identify Phospholipases A₂ that Regulate Docking/Priming in Calcium-Triggered Exocytosis.

Authors: Deepti Dabral; Jens R Coorssen
Journal: Cells Date: 2019-04-02 Impact factor: 6.600

Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.

1. The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.

2. Influence of ATP and calcium on the cortical reaction in sea urchin eggs.

Review 3. Calcium control of exocytosis and endocytosis in bovine adrenal medullary cells.

4. Fusion complex formation protects synaptobrevin against proteolysis by tetanus toxin light chain.

5. Cortical vesicle exocytosis in isolated cortices of sea urchin eggs: description of a turbidometric assay and its utilization in studying effects of different media on discharge.

6. Release of granule contents from sea urchin egg cortices. New assay procedures and inhibition by sulfhydryl-modifying reagents.

7. A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion.

Review 8. Vesicle fusion from yeast to man.

9. Calcium-dependent exocytosis in an in vitro secretory granule plasma membrane preparation from sea urchin eggs and the effects of some inhibitors of cytoskeletal function.

10. Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. I. Discharge of vesicular contents across the planar membrane.

Review 1. Proteins involved in synaptic vesicle trafficking.

2. The Role of Phospholipase D in Regulated Exocytosis.

3. A new approach to the molecular analysis of docking, priming, and regulated membrane fusion.

4. Roles of cholesterol in vesicle fusion and motion.

5. Enhancement of the Ca(2+)-triggering steps of native membrane fusion via thiol-reactivity.

6. A kinetic analysis of calcium-triggered exocytosis.

7. Generation, annotation, evolutionary analysis, and database integration of 20,000 unique sea urchin EST clusters.

8. Observations of calcium dynamics in cortical secretory vesicles.

Review 9. Multiple roles for the actin cytoskeleton during regulated exocytosis.

10. Combined targeted Omic and Functional Assays Identify Phospholipases A₂ that Regulate Docking/Priming in Calcium-Triggered Exocytosis.