Literature DB >> 10513618

Molecular cloning and characterization of two cDNAs for Glycyrrhiza glabra squalene synthase.

H Hayashi1, A Hirota, N Hiraoka, Y Ikeshiro.   

Abstract

Two cDNAs (GgSQS1 and GgSQS2) encoding squalene synthase have been isolated from licorice, Glycyrrhiza glabra L., and characterized. The deduced amino acid sequence of GgSQS1 was 88%, 81%, 78%, 45-44%, and 45-41% identical to those of GgSQS2, Nicotiana, Arabidopsis, mammal and yeast squalene synthases, respectively. Squalene synthase activity was found in the cell-free extracts of Escherichia coli transformed with the recombinant plasmids for GgSQS1 and GgSQS2, respectively. Genomic Southern blot hybridization indicated that there are three squalene synthase genes in the licorice genome. Northern blot analysis showed that GgSQS2 mRNA is mainly expressed during the exponential growth phase of the cultured licorice cells.

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Year:  1999        PMID： 10513618     DOI： 10.1248/bpb.22.947

Source DB:  PubMed          Journal:  Biol Pharm Bull        ISSN： 0918-6158            Impact factor:   2.233


  16 in total

1.  Functional characterization and differential expression studies of squalene synthase from Withania somnifera.

Authors:  Neha Gupta; Poonam Sharma; R J Santosh Kumar; Rishi K Vishwakarma; B M Khan
Journal:  Mol Biol Rep       Date:  2012-06-21       Impact factor: 2.316

2.  Gene regulation patterns in triterpene biosynthetic pathway driven by overexpression of squalene synthase and methyl jasmonate elicitation in Bupleurum falcatum.

Authors:  Young Soon Kim; Jung Hyun Cho; Sangkyu Park; Jung-Yeon Han; Kyoungwhan Back; Yong-Eui Choi
Journal:  Planta       Date:  2010-10-30       Impact factor: 4.116

3.  Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin.

Authors:  Hikaru Seki; Satoru Sawai; Kiyoshi Ohyama; Masaharu Mizutani; Toshiyuki Ohnishi; Hiroshi Sudo; Ery Odette Fukushima; Tomoyoshi Akashi; Toshio Aoki; Kazuki Saito; Toshiya Muranaka
Journal:  Plant Cell       Date:  2011-11-29       Impact factor: 11.277

4.  Upregulation of phytosterol and triterpene biosynthesis in Centella asiatica hairy roots overexpressed ginseng farnesyl diphosphate synthase.

Authors:  Ok Tae Kim; Sun Hee Kim; Kiyoshi Ohyama; Toshiya Muranaka; Yong Eui Choi; Hyeon Yong Lee; Min Young Kim; Baik Hwang
Journal:  Plant Cell Rep       Date:  2010-02-27       Impact factor: 4.570

5.  EST analysis reveals putative genes involved in glycyrrhizin biosynthesis.

Authors:  Ying Li; Hong-Mei Luo; Chao Sun; Jing-Yuan Song; Yong-Zhen Sun; Qiong Wu; Ning Wang; Hui Yao; André Steinmetz; Shi-Lin Chen
Journal:  BMC Genomics       Date:  2010-04-28       Impact factor: 3.969

6.  Cloning and characterization of a squalene synthase gene from a petroleum plant, Euphorbia tirucalli L.

Authors:  Hidenobu Uchida; Hirofumi Yamashita; Masataka Kajikawa; Kiyoshi Ohyama; Osamu Nakayachi; Ryuji Sugiyama; Katsuyuki T Yamato; Toshiya Muranaka; Hideya Fukuzawa; Miho Takemura; Kanji Ohyama
Journal:  Planta       Date:  2009-03-13       Impact factor: 4.116

7.  Molecular cloning and differential expression analysis of a squalene synthase gene from Dioscorea zingiberensis, an important pharmaceutical plant.

Authors:  Yun Ye; Runfa Wang; Liang Jin; Junhao Shen; Xiaotong Li; Ting Yang; Mengzhuo Zhou; Zhifan Yang; Yongqin Chen
Journal:  Mol Biol Rep       Date:  2014-07-05       Impact factor: 2.316

8.  Characterization of Squalene synthase gene from Chlorophytum borivilianum (Sant. and Fernand.).

Authors:  Shikha Kalra; Sunil Kumar; Neha Lakhanpal; Jagdeep Kaur; Kashmir Singh
Journal:  Mol Biotechnol       Date:  2013-07       Impact factor: 2.695

9.  Licorice beta-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin.

Authors:  Hikaru Seki; Kiyoshi Ohyama; Satoru Sawai; Masaharu Mizutani; Toshiyuki Ohnishi; Hiroshi Sudo; Tomoyoshi Akashi; Toshio Aoki; Kazuki Saito; Toshiya Muranaka
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-08       Impact factor: 11.205

10.  Virus-induced gene silencing of the two squalene synthase isoforms of apple tree (Malus × domestica L.) negatively impacts phytosterol biosynthesis, plastid pigmentation and leaf growth.

Authors:  Sandra M Navarro Gallón; Carolina Elejalde-Palmett; Dimitri Daudu; Franziska Liesecke; Frédéric Jullien; Nicolas Papon; Thomas Dugé de Bernonville; Vincent Courdavault; Arnaud Lanoue; Audrey Oudin; Gaëlle Glévarec; Olivier Pichon; Marc Clastre; Benoit St-Pierre; Lucia Atehortùa; Nobuyuki Yoshikawa; Nathalie Giglioli-Guivarc'h; Sébastien Besseau
Journal:  Planta       Date:  2017-03-27       Impact factor: 4.116
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