Literature DB >> 10512789

Towards molecular farming in the future: transient protein expression in plants.

R Fischer1, C Vaquero-Martin, M Sack, J Drossard, N Emans, U Commandeur.   

Abstract

Molecular farming in plants can be achieved by stable or transient expression of a recombinant protein. Transient expression of recombinant proteins in plants can rapidly provide large amounts of the proteins for detailed characterization. It is fast, flexible and can be carried out at field scale using viral vectors, but it lacks the increases in production volume that can be achieved easily with stable transgenic crops. This review article focuses on discussing the applications of transient expression using viral vectors, biolistic methods or agroinfiltration.

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Year:  1999        PMID: 10512789

Source DB:  PubMed          Journal:  Biotechnol Appl Biochem        ISSN: 0885-4513            Impact factor:   2.431


  30 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  2006-09-25       Impact factor: 11.205

2.  Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules.

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3.  Plant-produced cottontail rabbit papillomavirus L1 protein protects against tumor challenge: a proof-of-concept study.

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Journal:  Clin Vaccine Immunol       Date:  2006-08

Review 4.  Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules.

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Journal:  Plant Biotechnol J       Date:  2011-11-08       Impact factor: 9.803

5.  Transient expression of anti-VEFGR2 nanobody in Nicotiana tabacum and N. benthamiana.

Authors:  Mostafa Modarresi; Mokhtar Jalali Javaran; Masoud Shams-Bakhsh; Sirous Zeinali; Mahdi Behdani; Malihe Mirzaee
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6.  Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity.

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7.  In planta production of a candidate vaccine against bovine papillomavirus type 1.

Authors:  Andrew J Love; Sean N Chapman; Slavica Matic; Emanuela Noris; George P Lomonossoff; Michael Taliansky
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8.  Expression of recombinant cellulase Cel5A from Trichoderma reesei in tobacco plants.

Authors:  Megan Garvey; Johannes Klinger; Holger Klose; Rainer Fischer; Ulrich Commandeur
Journal:  J Vis Exp       Date:  2014-06-13       Impact factor: 1.355

9.  Transient Expression of cor Gene in Papaver somniferum.

Authors:  Bahman Hosseini; Farajollah Shahriari-Ahmadi; Haleh Hashemi; Mohammad-Hasan Marashi; Mahdi Mohseniazar; Alireza Farokhzad; Masoume Sabokbari
Journal:  Bioimpacts       Date:  2011-12-26

10.  A geminivirus AYVV-derived shuttle vector for tobacco BY2 cells.

Authors:  D Tamilselvi; G Anand; S Swarup
Journal:  Plant Cell Rep       Date:  2004-04-07       Impact factor: 4.570

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